The nitrate in water was analyzed  using a spectrophotometric method. The absorbances of the standards and the unknown sample were obtained at 550 nm. Solution                    Absorbance 2.00 µM                          0.065 6.00 µM                          0.205 10.00 µM                       0.338 14.00 µM                       0.474 18.00 µM                       0.598 Unknown                       0.402  Write the equation of the line What is the standard deviation of the calibration curve?

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UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or  ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.