n determining the concentration of a sample X, it was found that it has maximum absorbance at 275 nm. Compound X, has molar absorptivity at 275 nm of 8400 L / mol.cm. Using a spectrophotometer with a 1 cm cuvette, an absorbance of 0.70 was found. What is the concentration of compound X in the sample?

n determining the concentration of a sample X, it was found that it has maximum absorbance at 275 nm. Compound X, has molar absorptivity at 275 nm of 8400 L / mol.cm. Using a spectrophotometer with a 1 cm cuvette, an absorbance of 0.70 was found. What is the concentration of compound X in the sample?

Macroscale and Microscale Organic Experiments

7th Edition

ISBN:9781305577190

Author:Kenneth L. Williamson, Katherine M. Masters

Publisher:Kenneth L. Williamson, Katherine M. Masters

Chapter14: Ultraviolet Spectroscopy, Refractive Indices, And Qualitative Instrumental Organic Analysis

Section: Chapter Questions

Problem 4Q

See similar textbooks

Similar questions

A standard containing 20 mg/mL of compound P is placed in a 1 cm cuvette. The absorbance of the standard is 1.20 at 600 nm. A sample containing Compound P has an absorbance of 0.50. What is the concentration of this sample?
2) In a laboratory study, a calibration was performed using NH4C1 to analyze ammonium spectrophotometrically. The results of the study are given below. According to this, when the 5.0 mL sample containing ammonium is completed to 50 mL with pure water, find the amount of NH4 in the sample whose absorbance was found as 0.12 according to the table. (N: 14 g / mol, H: 1 g / mol, Cl: 35.5 g / mol). Concentration (mg/L) Absorbance 0.01 5 0.05 7.5 0.1 10 0.15 12 0.18
What value of absorbance corresponds to 45.0% T?
A colored ion solution has a concentration of 0.200 M with a measured absorbance A = 0.880. Another ion solution made of the same chemicals has an absorbance A = 0.172. What is the concentration of this unknown sample solution?
Compound X was dissolved in a 5-mL volumetric flask. A 1.00-mL aliquot of the compound was placed in a 10-mL volumetric flask and diluted to the mark. The solution was read in a UV-Vis spectrophotometer and an absorbance of 0.427 in a 1.000-cm cuvette was observed at 340 nm. Solve the following question with complete solution Data for Compound X: Molecular mass = 292.16 g/mol Molar absorptivity (ɛ) at 340 nm= 6130 M -1 cm -1 1. How many milligrams of compound X were used to make the 5-mL solution?
0.122 mg of Crystal Violet you synthesized was dissolved in 100 ml of solvent. The absorbance was found to be 0.209, what is the purity of the Crystal Violet you synthesized?
Compund AFK has a maximum absorbance of 650 nm. Its molar absorptivity at 650 is 8400 M−1cm−1 and the path length is 1 cm. Using a spectrophotometer, determine the concentration of Compound AFK it is absorbance at 650 nm is 0.700 ? 8.33x10-5 mol/L 1.20 x104 mol/L 1.08 x10-3 mol/L 7.74 x10-2 mol/L
Why it is important to measure the absorbance in the range of 0.4 to 0.9.
ion 7 of 24 A 6.91 x 10- M sample of compound X in a solvent has an absorbance of 0.229 at 528 nm in a 1.000 cm cuvette. The solvent alone has an absorbance of 0.052. What is the molar absorptivity of compound X? M-1.cm-1 € = A different sample of compound X in the same solvent has an absorbance of 0.504 at 528 nm when measured with the same cuvette. What is the concentration of this second sample? mol X [X] =
Blue Blue dye stock solution Absorbance at 630 nm Calibration curve Part 1: 0.1944 M 0.00697 y = 0.0682x A purple solution is prepared by mixing 5.00 mL of the blue dye solution with 6.75 mL of red dye solution. The calibration curve for each color is provided. Part 2 out of 3 (a) Determine the theoretical molar concentration of each dye in the mixture. [Blueltheoretical = 8.27 x 10-2 [Red] theoretical = 1.16 [Bluelexperimental [Redlexperimental " *10-1 M M x 10 (b) Using the absorbance and calibration curve for each dye, calculate the experimental molar concentration of each dye in the mixture. x 10 Red Red dye stock solution Absorbance at 500 nm Calibration curve 0.2015 M 0.00193 y = 0.0170x M M
Ni2+ appears green in solution, so its concentration can be determined spectrophotometrically. A student measured the absorbances of several solutions of known Ni2+ concentration and created a plot of absorbance vs. [Ni2+]. The equation of the best-fit line for the calibration curve was determined to be y = 1.45x + 0.00162. What would be the concentration of a Ni2+ solution that has a measured absorbance of 0.560?
A blue product absorbs light at 590 nm (ε590nm = 7,600 M-1 cm-1). Its concentration was too concentrated to determine an accurate absorbance reading. Therefore, the solution was diluted in the following manner before reading it's absorbance. 0.500 mL was added to 1.000 mL of water to make "Dilution 1". Then 0.700 mL of "Dilution 1" was added to 3.000 mL of water to make "Dilution 2". The absorbance reading of "Dilution 2" was determined to be 0.220 when using a 1 cm pathlength. Determine the millimolar (mM) concentration of the blue product in the enzyme reaction. Indicate the concentration to the precision of 3 decimal places. ANS: 0.078 nM. Would like some steps on how to get this answer. Thanks.