The free energy released by the hydrolysis of ATP under standard conditions is −30.5 kJ/mol. If ATP is hydrolyzed under standard conditions exceptat pH 5.0, is more or less free energy released? Explain.

A particular reaction has a ΔG‡ of 37.0 kJ mol-1. In the presence of an enzyme, the same reaction has a ΔG‡ of 5.70 kJ mol-1. Calculate the value of ΔΔG‡ in kJ mol-1.

Consider the following reaction and its equilibrium constant: 12(g) 21(g) Kp = 0.209 atm A reaction mixture contains 0.89 atm 12 and 1.77 atm I. Which of the following statements is TRUE concerning this system?

Describe how you would make the following solution containing two enzymes.             1 mL total volume in Buffer X with 10nM of enzyme A and 50nM of enzyme B               You are given a solution of enzyme A at 1 mg/mL and a solution of enzyme B at 1 mg/mL.  The molecular weight of enzyme A is 75,000 g/mol and the molecular weight of enzyme B is 130,000 g/mol.

The ΔG°′ value for glucose-1-phosphate is -20.9 kJ/mol. If glucose and phosphate are both at 4.8 mM, what is the equilibrium concentration of glucose-1-phosphate?

The formation constants at 25°C for Fe(CN)4-6 and Fe(EDTA)2– are 1.00 x 1037 and 2.10 x 1014, respectively. Answer the questions below. 1) Calculate K under standard conditions for the reaction Fe(EDTA)2−(aq) + 6CN−(aq) ----> Fe(CN)4−6​(aq) + EDTA4−(aq) 2) Calculate ΔG° for the reaction. (kJ/mol)

A particular reaction has a ΔG‡ of 30.0 kJ mol-1 at 25.0 °C. In the presence of an enzyme, the same reaction has a ΔG‡ of 1.50 kJ mol-1 at the same temperature. Calculate the rate enhancement of this enzyme. (R = 8.3145 J mol-1 K-1)

In many biochemical reactions which involves the formation of an enolate intermediate, the carbonyl oxygen of the substrate is coordinated to a divalent metal ion (usually zinc or magnesium) in the active site. Explain with structural drawings, how this ion-dipole interactions affect the acidity of the a-protons?

Calculate theequilibrium constant and thefree energy of (Mg)ATP hydrolysis in a closed system in which concentrations of ATP, ADP and Piare, respectively, 3.5, 1.0, and 5 mMand 25 degrees Celsius. Is the reaction spontaneous?R = 8.314 J/(deg Kelvin-mol).

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