The diagram below shows that of a plasmid containing genes that confers erythromycin (Erm®) and ampicillin (Amp®) resistance to bacteria that expresses these genes. On the outer portion of the diagram are the labels of some of the different restriction sites available for the insertion of foreign DNA. Part Scal Erm" plas02 EcoRI Pu (4.361bp) EcoR V BamHI Amp Sall651 The DNA segment that codes for the protein insulin was inserted into the plasmid using the restriction site BamH I. Which of the following is true if bacteria were able to successfully incorporate a plasmid with the insulin gene insert? The bacteria will not have any resistance to ampicillin or erythromycin. O The bacteria will exhibit resistance to both ampicillin and erythromycin. The bacteria will exhibit resistance to ampicillin but not to erythromycin. O The bacteria will exhibit resistance to erythromycin but not to ampicillin. O The bacteria will randomly exhibit resistance to either ampicillin or erythromycin, but not to both.

Q: 5' UTR OP This region is specific sequence surrounding the start codon. OK OS OT 5' and 3' UTR LEU2…

A: In bacteria, there is a small circular double-stranded extra-chromsomal DNA that can replicate…

Q: In the genotype presented (genomic plus plasmid genes), which of the following statements Is…

A: Lac operon codes Lac Z,Y,A genes which are structural genes encoding Beta galactosidase,Permease,…

Q: You have cloned a gene that codes for phosphoglycerate mutase from Leishmania mexicana (Lm- PGAM)…

A: Gene cloning is the technique to produce the exact copies of the desired gene by utilizing genetic…

Q: Imagine that you completed your ligation of the gRNA insert (annealed oligos) into cut pML104 vector…

A: Nuclear localization signals (NLS) is importnat because it helps to transport Cas9 into the nucleus…

Q: You are interested in synthesizing a variant of Synechocystis sp. pcc 7803 ferredoxin having…

A: Ferredoxins are a group of low-molecular-weight, acidic, non-heme, soluble iron-sulfur electron…

Q: Provide 2 diagrams to illustrate the 2 potential recombinant plasmids,

A: The question here states about the concerned recombination of cloned plasmids and their…

Q: Plasmid is  3.6kb large. It is digested with an enzyme that has restriction sites at  1,152 and…

A: Plasmids are circular DNA found in bacteria. They are extra chromosomal materials and are highly…

Q: EboV RNA from Guinea Pig EboV RNA from Guinea Pig GAU ACG UUC GUC AAU EboV DNA CTA TGC AAG CAG TTA…

A: Given sequence of EboV strain 1 is CTA TGC AAG CAG TTA and strian 2 is TCA TGT CAG CAA CTA.

Q: Which of the delivery techniques mentioned in Table 2 can be used to best transfer recombinant…

A: * They are many techniques for delivering the recombinant plasmids into the recipient cells. *…

Q: 5' 3' ORF1 ORF2 ORF3 ORF4 ORF5 1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and…

A: The production of RNA from DNA is known as transcription that occurs within the cytoplasm of…

Q: EboV RNA from Guinea Pig EboV RNA from Guinea Pig GAU ACG UUC GUC AAU EboV DNA CTA TGC AAG CAG TTA…

A: A missense mutation is a mistake in the DNA which results in the wrong amino acid being incorporated…

Q: In gene cloning, a vector is required to transform the gene of interest into host cell. State the…

A: In gene cloning, a vector is required to transform the gene of interest into the host cell. The…

Q: For pGLO plasmid that contains araC gene and Ampr gene, what are the names of their promoters and…

A: pGLO plasmid  pGLO plasmid is a 5371bp genetically engineered plasmid to create genetically modified…

Q: During plasmid extraction, after the addition of acetate-containing neutralization buffer the large…

A: Plasmid extraction is a method for the isolation of DNA. In this method, purified plasmids are…

Q: The transfection reagent used to introduce plasmids to the HEK293 cells was a lipid reagent. Why is…

A: Transfection is the incorporation of DNA, RNA, or proteins in to the eukaryotic cells for the…

Q: Many resistance mechanisms are encoded on plasmids. These mechanisms are of great clinical…

A: BLAST is a bioinformatics tool that enables us to identify a gene if we know its DNA sequence or the…

Q: For Figure 15-7, draw out a series of steps that could explain the origin of this large plasmid…

A: We need to provide the process leading to the origin of a large plasmid containing various…

Q: 5 μL of plasmid DNA (pUC 19) was added to 50ul of CaCl2 competent E. coli that was then added 250 μL…

A: Introduction :- A plasmid is a small, circular, double-stranded DNA molecule that can replicate…

Q: Can you answer the last three q

A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction:…

Q: For a molecular cloning experiment using the bacterial strain E. coli K12 is used to insert the gene…

A: Recombinant DNA technology uses enzymes and a variety of laboratory procedures to modify and isolate…

Q: Hindl EcoRI ECORV BamHI 373 Salt Pstl. tet amp Plasmid 1000 DBR322 4361 be ori Ndel you were going…

A: Restriction enzymes or restriction endonucleases/exonucleases are enzymes capable of recognizing a…

Q: After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that…

A: Bacterial transformation is a process through which bacteria can take up foreign DNA. Transformation…

Q: During plasmid extraction, after the addition of acetate-conta in ing neutralization buffer the…

A: Plasmids are extrachromosomal DNA in the bacterial cells. They are double stranded circular DNA,…

Q: How many restrictions sites are present in the EcoRI. Calculate the number of restriction sites for…

A: Restriction endonucleases or restriction enzymes are enzymes that cleave or cut nucleic acids at…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: ori Clo fcoR v OFP Below is the plasmid map of pGLO which contains the Green Fluorescent Protein…

A: pGLO:   pGLO is an engineered plasmid that contains genes for beta- Lactamase.  Beta-lactamase…

Q: Assume that a circular plasmid is 3200 base pairs in length and has restriction sites for HindIII…

A: HindIII is a Haemophilus influenzae type II site-specific deoxyribonuclease restriction enzyme that…

Q: When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with…

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Q: Why is it necessary to prepare positive and negative control reactions in restriction digestion of a…

A: In restriction digestion of a plasmid (circular DNA molecule), preparing positive and negative…

Q: dont provide handwriting solution ...

A: When dealing with circular DNA containing multiple EcoRI recognition sites, the digestion pattern…

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A: The restriction endonuclease is the enzymes that help in the deduction of the DNA from a specific…

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Q: Uncut - DNA Fragments produced B2 Sspl Enzyme(s) EcoR1 Smal used - 5.0 plasmid, how many times does…

A: Introduction :  The DNA-based structure known as a plasmid can exist and replicate on its own in…

Q: What role did the transposon play in the transfer of resistance?

A:

Q: For the plasmid below, list the origin and what antibiotic you would use for selection?

A: Plasmid is the extra chromosomal DNA present in prokaryotic cells. It contains information that…

Q: vector pUST, gene W, and the restriction cut sites. What are 3 cutting strategies for cloning gene W…

A: *Cloning means producing an organisms with identical DNA either by natural or through artificially .…

Q: Besides the pUC series, there are other plasmid vectors in the market such as pBR327 and pGEM-T.…

A: Cloning methods are used in biotechnology for various purposes. TA cloning is the technique that…

Q: The Ti plasmid, is often used for making transgenic plants. Where the plasmid is found?

A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…

Q: In Figure 5-19, how many different bacterial species areshown as having contributed DNA to the…

A: A plasmid is an extrachromosomal circular DNA molecule found in the bacteria which is able to…

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A: An auxotrophic mutant refers to the strain having a mutation that does not allow the strain to…

Q: Question: You conclude that all the blue colonies contain a plasmid

A: pUC is a plasmid cloning vector which is created by Joachim Messing and co-workers. Restriction…

Q: distinguish between bacterial cells that obtained the plasmid and those that did not

A: Bacteria are single celled organisms with a unique internal structure.

Q: is shown below. Draw a restriction map of a plasmid with the appropriate restriction sites based on…

A: Restriction digestion is the fragmentation(cutting) of DNA on very specific sites (restriction…

Q: You utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using…

A: Bacteria produce the restriction enzyme, a protein that cleaves DNA at certain locations.  The…

Q: You want to clone the gene Dik1 into a plasmid that will be used to generate a transgenic mouse. The…

A:

Q: A target gene for producing human insulin must be inserted into a plasmid before the transformation…

A: Option 2 is correct only 1 and 3 follows

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A: Exchange of F plasmid or fertility factor occurs from F+ , a donor to F- , which acts as the…

Q: Plasmid PRL27 is mobilizable. Given this information, and compared to pUC18, this plasmid must…

A: Conjugation is a process by which bacteria pass on genetic information from one cell to other. This…

Q: In an restriction enzyme experiment where Eco RI and  Hind III are used . What would happen if a…

A: Introduction  An isolated bacterial protein known as a restriction enzyme cleaves DNA at…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.