The cI protein in bacteriophage l (lambda) is a transcriptional regulator - name the promoters in the phage genome that are regulated by this protein, and give the effect of the cI protein on each promoter you name (i.e. is the cI protein an activator or a repressor for that promoter).

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: The Lac operon contains genes responsible for the transportation and breakdown of lactose. These…

Q: 12445 RD-Hom 4(167-277) A(194-277) A(97-138) A(97-167) 6 A(97-194) N595A 7890 A(619-723) HD 10…

A: A regulatory protein regulates the expression of a gene by binding with that gene through its DNA…

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: Operon acts as a single regulated unit having one or more structural genes, promoter gene, a…

Q: ne of the main lessons of this chapter is that several bacterial genes are often transcribed from a…

A: As given: Several bacterial genes are often transcribed from a single promoter into a large…

Q: The only way that information is extracted out of DNA is through the transcription of MRNAS that…

A: According to our guideline we can answer only the first question. So upload the second question…

Q: In the lac operon (below), how will expression of the genes lacZ and lacy be effect by a mutation in…

A: Lac operon includes the genes that code for the enzymes involved in the catabolism of lactose sugar.…

Q: Chromatin Immunoprecipitation (ChIP) experiments enable researchers to measure the levels of…

A: LPS (lipopolysaccharide) treatment results in the recruitment of the transcription factor NfkB and…

Q: Suppose you had a bacteriophage λ in which the gene encoding the cro protein had been mutated, so…

A: The mutation alters allele frequencies by constantly introducing new alleles, which can be…

Q: Which of the following statements is not true of both the LacI and TrpR repressor proteins?a) an…

A: The objective of the question is to identify the statement that is not true for both LacI and TrpR…

Q: Consider a mutant of E. coli that has an inactivating mutation in the gene for the catabolite…

A: The lac operon is a negatively inducible operon.

Q: Some prion-related diseases, such as familial fatal insomnia, areinherited. How would you expect the…

A: Familial fatal insomnia is a medical condition characterized by insomnia or trouble sleeping. This…

Q: E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a…

A: When a group of related genes exist under the control of a single promoter, it is referred to as an…

Q: Explain using leucine zipper motifs as an example, how protein-protein interactions between…

A: DNA is the genetic information that a cell obtains from its parents. DNA contains a specific…

Q: The mRNA used for the SARS-CoV-2 vaccine from Pfizer/BioNTech is generated in a cell-free system by…

A: The hereditary molecule deoxyribonucleic acid (DNA) transmits genetic information from one…

Q: A mutation in the repressor LacI causes it to lose the ability to bind its effector, allolactose.…

A: The question is asking about the effect of a mutation in the LacI repressor that causes it to lose…

Q: A eukaryotic cDNA was ligated to a phage DNA which was transfected into a bacterial host cell. The…

A: The cDNA is produced from the mature mRNA by the action of reverse transcriptase enzyme.

Q: In transcription Rho-dependent termination in bacteriophage is more common than in bacteria. True…

A: Transcription is defined as the synthesis of mRNA from the template strand of DNA. The RNA…

Q: A novel operon is controlled by two regulatory systems that function in a hierarchy. The first is…

A: An operon can be defined as the cluster of coordinately controlled and regulated genes. It is…

Q: Contrast the two types of transcription terminators in E.coli.

A:

Q: The protein NK2 is a transcriptional regulator that functions both as a repressor and activator. To…

A: A regulatory protein regulates the expression of a gene by binding with that gene through its DNA…

Q: Pre-initiation complex (PIC) is formed right before the initiation of genes, which of the following…

A: The preinitiation complex is a protein construct that forms in eukaryotic cells prior to…

Q: ptoph had a mutation on the repressor, not allowing it to bind with tryptophan. The repressor is…

A: Operons are proteins encoded together as a block that performs specific functions and are involved…

Q: Which promoter initiates which life cycle (lysogenic & Lytic)? Mention both types of promoters and…

A: Viruses reproduce by utilising a host cell to produce additional viruses, which subsequently burst…

Q: Explain the single operator site that is most important for repression.

A: Introduction: Operons are used by prokaryotes to control their genes. Numerous genes are under the…

Q: For the following types of transcriptional control, indicate whether the protein produced by the…

A: An inducible operon is one which is not transcribed. For transcription of the molecule, it requires…

Q: Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC,…

A: Question 1:The genB gene's translation would be prematurely stopped by a nonsense mutation in the…

Q: Tryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too…

A: Attenuation is a mechanism of control of the tryptophan (Trp) operon that occurs when the level of…

Q: The following diagram show what is required for an active promoter of a gene of interest, where:A1 =…

A: Based on the given diagram, here are the predictions:Chromatin conformation: EuchromatinMethylation…

Q: Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC,…

A: Question 1: B. The amino acid is bound to the regulator, causing the regulator to bind to the…

Q: A particular mutation in the bacterial sigma factor allows this protein to bind RNA polymerase but…

A: Answer - Option A - It will prevent the transcription termination exerted by the Rho protein

Q: Bacteria with a lac phenotype are unable to use lactose because of some defect in expressing the…

A: Answers - 1) False 2) True 3) True  There are 3 structural genes in lac operon - lac Z, lac Y and…

Q: You have cells that are mutant in CAP protein such that it cannot bind to CAMP. For these mutant…

A: Cap protein- In solution, the catabolite activator protein (CAP, also known as cAMP receptor…

Q: For the lac operon, when glucose is absent and lactose is present ___________       None of the…

A: Question - For the lac operon, when glucose is absent and lactose is present ___________…

Q: At 2:00 pm, you measured intracellular arginine at 100M. After addition of 200M of arginine to the…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: For the trp operon, determine whether genes will be expressed (on) or silenced (off) given the…

A: Gene is a basic unit of hereditary information or data that fills a fixed position on a chromosome.…

Q: Which of the following accurately describes the function of HOTAIR? O It is a long non-coding RNA…

A: Option 1 is the correct answer.

Q: Calculate the concentration of lac repressor, assuming that one molecule is present per cell. Assume…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: In bacteria, genes that are often used together are controlled by a single promoter. Explain why…

A: Gene regulation refers to the numerous processes that regulate which genes are expressed and the…

Q: One of the main lessons of this chapter is that several bacterial genes are often transcribed from a…

A: As given: Several bacterial genes are often transcribed from a single promoter into a large…

Q: For the lac operon, when glucose is absent and lactose is present. CAMP-CAP is bound to the promoter…

A: The lac operon is a genetic regulatory system in bacteria that controls the expression of genes…

Q: Describe the promoter structure in prokaryotes and eukaryotes.

A: A gene's "promoter", which serves as a control point for controlled gene transcription, is a…

Q: Regarding the tryptophan operon in bacteria, in the presence of tryptophan, tryptophan binds to the

A: Q. Regarding the tryptophan operon in bacteria, in the presence of tryptophan, tryptophan binds to…

Q: The bacterial operon (a set of co-expressed genes) shown below contains one promoter (green), two…

A: In a bacterial operon a single mRNA containing multiple genes are transcribed from a single…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.