The cI protein in bacteriophage l (lambda) is a transcriptional regulator - name the promoters in the phage genome that are regulated by this protein, and give the effect of the cI protein on each promoter you name (i.e. is the cI protein an activator or a repressor for that promoter).

Describe two examples of regulators that acted on transcription at steps after initiation. Of the two examples, one must be from a phage system and the other from a bacterial biosynthetic operon.

Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented.   Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent.                                    Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow                        Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.

The fictional bacteria Plumbumbacter aurus converts the lead ions to gold ions and uses the resulting electrons to create ATP. aurus has the midas operon which includes the promoter (midasP), the operator (midasO), and eight genes that encode proteins involved in converting lead to gold. The midas operon is regulated by the protein encoded by the midasR gene and the presence of gold ions. When the concentration of gold ions is low, the midas operon is transcribed and the bacteria convert lead into gold. When the concentration of gold is high, the midas operon is off. To better understand regulation of the midas operon, you created P. aurus mutants. The midasR- mutant has a nonfunctional midasR, the midasO- mutant has a nonfunctional midasO, and the midasP-mutant has a nonfunctional midasP. Based on the data below, the midas operon has what type of transcription control? (negative inducible, negative repressible, positive inducible, positive repressible)  Strain Produces Gold in…