The cI protein in bacteriophage l (lambda) is a transcriptional regulator - name the promoters in the phage genome that are regulated by this protein, and give the effect of the cI protein on each promoter you name (i.e. is the cI protein an activator or a repressor for that promoter).

Q: All of the following statements about the repressor of the lac operon of E. coli are true EXCEPT…

A: The lac operon is a clustered group of related genes that are transcribed as a single unit. These…

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: Operon acts as a single regulated unit having one or more structural genes, promoter gene, a…

Q: Two bacterial genes are transduced simultaneously. What does this suggest about the location of the…

A: Genetic recombination in bacteria occurs by transformation, conjugation and transduction.

Q: What would happen if the operator sequence of the trp operon contained a mutation that prevented the…

A: Trp Operon is an operon that codes for the components for the production of Tryptophan, it consists…

Q: The process in which the two-dimensional structure of RNA from the L region of an operon can either…

A: The strategy of creating an RNA duplicate of a grouping is transcription. The transcript, known as…

Q: Suppose you had a bacteriophage λ in which the gene encoding the cro protein had been mutated, so…

A: The mutation alters allele frequencies by constantly introducing new alleles, which can be…

Q: Operon X produces three enzymes that convert Compound A to Compound B. The R gene makes the…

A: Gene expression is given on the basis of RNA. It is a process through which there is turning on of…

Q: How can the bacteriophage T7 promoter be used to controlexpression of a eukaryotic gene in…

A: Expression vectors are designed to control the expression of particular cloned genes. The native…

Q: Which of the following statements is/are TRUE for the promoters? The Pribnow box and TATA box are…

A: A promoter is a short region of DNA upstream of  gene where RNA Polymerase recognizes the start site…

Q: According to the operon model, for the synthesis of an inducible enzyme to occur, the end-product…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Which of the following DNA regions Is NOT Involved in gene expressi regulation in eukaryotes?

A: Ans- Operators are not involved in the Eukaryotic Gene Expression. Eukaryotic protein-coding genes…

Q: Attardi et al (1963) found out that lactose operon mRNA synthesis in E. coli increased with the…

A: Introduction Operon is a genetic regulatory system found in bacteria and their viruses that clusters…

Q: Some organisms have mechanisms in place that will override transcription termination. One such…

A: The HIV-1 (Human Immunodeficiency virus-1) Tat (transactivator of transcription) gene codes for a…

Q: Components necessary for regulating the /trp/ operon do NOT include

A: Answer - Option E - CAP protein

Q: For each of the 4 regulatory states of the lac operon shown in the following diagram, answer the…

A: Genes active in lactose synthesis are found in E. coli's lac operon. When lactose is present but…

Q: If an abnormal repressor protein could still bind allolactose, but thebinding of allolactose did not…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…

Q: An enhancer, located upstream from a gene, has the followingsequence:   5´–GTAG–3´   3´–CATC–5´This…

A: Deoxyribonucleic acid (DNA) is a genetic material and it carries genetic information from one cell…

Q: Glucose absent, lactose absent     Glucose absent, lactose present     Glucose present, lactose…

A: An operon is found in bacteria and viruses as a genetic regulatory system. The gene coding for…

Q: ptoph had a mutation on the repressor, not allowing it to bind with tryptophan. The repressor is…

A: Operons are proteins encoded together as a block that performs specific functions and are involved…

Q: Which of the following describes the lac operon in E. coli when lactose and glucose is present in…

A: The lac repressor acts as a lactose sensor. It normally blocks transcription of the operon, but…

Q: ferR ferP. ferS ferI ferZ KEY: O = operator P = promoter Boxes = structural genes Arrows =…

A: An operon is the unit of bacterial gene expression and regulation. The operon contains the…

Q: What is the role of arabinose in production of GFP protein from pGLO-transformed bacteria? O When…

A: Given: The role of arabinose in production of GFP protein from pGLO transformed bacteria.

Q: An enhancer, located upstream from a gene, has the following sequence: 5’-ACGA-3’…

A: answer given below..

Q: In the lac operon, regulation of gene expression by CAP-CAMP is a type of O a. positive repressible…

A: Lac operon can be defined as the set of  constitutive genes with a single promoter that encodes for…

Q: The MRNA for a mammalian peptide that enhances electrolyte transfer to reduce paralysis was spliced…

A: The cells are of two types eukaryotic and prokaryotic cells. The primary difference between the cell…

Q: (MC) What is the function of lactose in regulation of the lac operon? 1. activates a repressor…

A: Operons are a group of enzymes and regulatory sequences.

Q: Which of the following is FALSE? Sigma domain 3.2 makes direct contact with the 5'-end of the…

A: Gre proteins of prokaryotes are transcription elongation factors that promote an endogenous…

Q: A bacterial cell undergoes a change and the two Trp codons of the tryptophan leader sequence are…

A: Tryptophan operon is an example of repressible operon which remain transcriptionally active and…

Q: You are examining a set of genes from a phage that are transcribed late in infection. You know that…

A: Transcription: It is a process of synthesis of mRNA transcript from the double-stranded DNA as a…

Q: Which of the following lambda phage proteins is a transcription activator? Select one: Cl protein а.…

A: Biogeochemical processes, gene modification, and bacterial diversity are all dependent on…

Q: For the wildtype E. coli two sugars influence the level of transcription of the lactose operon. Fill…

A: Sugars in the growth media cAMP levels (High or Low) Positive regulation occurs (Yes…

Q: Regarding the tryptophan operon in bacteria, in the presence of tryptophan, tryptophan binds to the

A: Q. Regarding the tryptophan operon in bacteria, in the presence of tryptophan, tryptophan binds to…

Q: For each of the following conditions in the Lac Operon, state whether transcription will occur or…

A: The lac operon of E. coli contains genes responsible  in lactose metabolism. It is  expressed only…

Q: A bidirectional enhancer has the following sequence: 5′–GTCA–3′ 3′–CAGT–5′ Which of the following…

A: Regulatory elements are the elements that regulate the process of transcription. These are of two…

Q: For transcription of middle promoters in T4 phage, the anti-sigma factor, AsiA, will а. bind -10…

A: The bacteriophage T4 Alt gene product is a component of the phage head and, together with the phage…

Q: Contrast the effects of mutations in genes encoding sporulation-specific sigma factors with…

A: Introduction Mutation in sigma factors:- Sigma factors are subunits of all bacterial RNA…

Q: What is the role of catabolite activator protein (CAP) in the lactose operon of E. coli?

A: Lac operon Lac operon is a group of protein that code for enzyme β-galactosidase. The function of…

Q: The fictional bacteria Plumbumbacter aurus converts the lead ions to gold ions and uses the…

A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…

Q: An enhancer, located upstream from a gene, has the following sequence:    5’-ACGA-3’…

A: In the field of molecular biology, an enhancer is referred to as the short (50–1500 bp) region of…

Q: Theara operon is an inducible operon that controls the production of the sugar arabinose. When…

A: An operon is a group of genes that are transcribed together and are under the influence of a single…

Q: The lac operon is a negative inducible system and allolactose will bind to the regulator protein…

A: In normal state, the lac operon is generally turned off. A repressor protein during "off" stage,…

Q: Which subunit of bacterial RNA polymerase interacts with the -10 region of the promoter?

A: There are two questions on Transcription.

Q: For each of the following types of transcriptional control, indicate whether the protein produced by…

A: An inducible operon is one which is not transcribed. For transcription of the molecule, It requires…

Q: In the: Mutation of the regulatory region for a repressor protein in the lac operon. Explain: (a)…

A: Operon  It is a set of multiple gene that is controlled by single operator.

Q: In the presence of arabinose sugar and absence of glucose, which of the following conditions may be…

A: Arabinose could also be a five-carbon sugar that happens naturally and can be used as a result of…

Q: which of the following statements about the A pahge are TRUE, which ones are FALSE? Phage genes are…

A: Phage genes are repressed during the lysogenic state. Answer) False The phage has a temperate…

Q: An E. coli cell as the following genotype for the lac operon system:  I- OC Z+ / F’ I+.  Which term…

A: The presence I+ repressor is dominant to the absence of a repressor I-. Oc mutants:-  these are the…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.