The amino acid glycine is often used as an ingredient in buffers for biochemistry experiments. The amino group of glycine has a pKa of 9.6. Glycine exists in either a protonated form (-NH3*) or a free base (-NH₂). a) In what pH range can glycine be used as an effective buffer? b) In a 0.1M solution of glycine at pH 9.0, what fraction has its amino group in the protonated form? c) When 99% of the glycine is in the protonated from, what is the numerical relation between the pH of the solution and the pKa of the amino group?

Determine the net charge on the following pentapeptide "Lys-Ala-His-Asp-Ser" at: a. pH 1.0 b. pH 10.1

One liter of a 0.1 M Tris buffer (pKa of Tris = 8.3, see Table 2.4) is prepared and adjusted to a pH of 2.0. A) What are the concentrations of the conjugate base and weak acid at this pH?Answer with 2 significant digits. I have solved for [HA] = 0.10 Can you please help solve for [A-] = ?

2.1 Estimate the solubility of calcium carbonate at 25°C in the presence of 0.02M CaCl2(aq). 2.2 Will BaSO4 precipitate from a solution containing 2.5 x10 M Ba*2, barium ion, if enough soluble salt, Na2SO4, is added to make the solution 2.5 x104 M in SO42?

Hemoglobin is considered to be a tetrameric complex with a 64 kDa (α β)2. When attempting to purify hemoglobin, we must first purify the α and β monomers (about 16 kDa each) to prepare the tetramer. This is formed from the dimer intermediate: 2 α + 2 β -> 2 αβ -> (α β)2. 1. The graph given represents a size-exclusion chromatogram after the refolding of the hemoglobin tetramer. This process is not 100% efficient, so we may have leftovers of dimers and monomers. In the graph given, can we label the peaks given as a tetramer, dimer, or monomer?

The structure of ephedrine isgiven, The pKa for ephedrine is 9.6. Answer the following questions about aqueous solutions of ephedrine. a. Draw the structure of the principal chemical form of ephedrine that results when it isdissolved in water.b. Draw the structure of the principal chemical form of ephedrine that results when it is dissolved in a buffer at a physiologically relevant pH of 7.4.c. Calculate the pH that results when 0.3 mole of ephedrine is dissolve in 2.0 L of 0.05 M perchloric acid (HClO4).

Phosphate buffers are commonly used to mimic biological systems. Given that phosophoric acid is a triprotic acid with three pKas (2.12, 7.21, and 12.32), why do you think this is? What are the dominant buffering compounds present in a phosphate buffer near physiological pH?

0.03 The uv spectrum of a protein solution shows A280 nm = 0.43 and A260 nm = What is the approximate protein concentration in mg/mL?

Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4 * 10° positively charged amine groups that can adsorba negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. 2. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the…

Amino acid structure For any ionizable group, indicate the pka value and draw the structure that would be expected at the pH inside the cell (~7.4).