Shown are results of clinical FACS analysis of a normal individual and a patient with X-linked agammaglobulinemia (XLA). Blood lymphocytes from these two patients were incubated with antibodies that recognize the B-cell marker CD19 and the T-cell marker CD3 and then sorted by flow cytometry. Which patient is healthy? Please select.

Each of the following statements is false. Please correct. a. Mature T cells are found only in the lymph nodes and spleen b. The pluripotent stem cell is one of the most abundant cell types in the bone marrow c. There are no stem cells in blood d. Activation of macrophages increases their expression of MHC class I molecules, allowing them to present antigen to CD4+ TH cells more effectively e. B cells develop in the thymus f. Lymphoid follicles are present only in the spleen and lymph nodes g. The FRC guides B cells to follicles h. Infection has no influence on the rate of hematopoiesis i. Follicular dendritic cells can process and present antigen to T lymphocytes j. Dendritic cells arise only from the myeloid lineage k. All lymphoid cells have antigen-specific receptors on their membrane l. All vertebrates generate B lymphocytes in bone marrow m. Only mammals have a thymus n. Jawless vertebrates do not have lymphocytes

Shortly discuss the above antibodie structure? Please discuss at your own words . Discussion should be to the point (specific 5-6 lines).

1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge  true/false 3)  PBST  is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme  A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8