RESTRICTION ENZYME MAPPING Predict the result

Q: Restriction Mapping. Single and double digestion of plasmid pMCS326 were performed using the…

A: Restriction mapping is a technique for mapping an unknown section of DNA by breaking it apart and…

Q: Elaborate on three (3) lysis methods used to disrupt bacterial cells.

A: Cell lysis or cell disturbance is a strategy wherein the external limit or cell layer is separated…

Q: DNa Mapping using Restriction enzymes lab: We will be aliquoting and delivering 5 μl of enzyme to…

A: A restriction enzyme, also known as restriction endonuclease is an enzyme that cleaves DNA into…

Q: (-) A B C DEF G TE G Developmental Stage Legend: A- 1" instar D- 4 instar G- adult B- 2nd instar E-…

A: Electrophoresis is the technique to isolate various band sizes of DNA (deoxyribonucleic acid),…

Q: Which temperature is Taq polymerase's optimal temperature O 20 degrees C 72 degrees C 55 degrees C O…

A:

Q: For each of the following items, describe what it is explain the necessity for each in cloning…

A: Genetic engineering is the application of technology in the manipulation of genetic information.…

Q: Do virtual restriction digests of 300ng pGLO plasmid using HinDIII, EcoRI, and XhoI separately, and…

A: DNA restriction enzymes cleaves the DNA at specific sites. These recognition sites have DNA…

Q: A mutant E. coli strain has been discovered that has a non-functional lac operon. It's genotype is…

A: In order for the mutant E. coli strain to produce β-galactosidase, a mutation would be needed in the…

Q: how an ion-selective field effect transistor (ISFET) can be used as a biosensor to detect DNA in…

A: An ISFET biosensor can be used to detect DNA in solution by detecting changes in the pH of the…

Q: what is the difference between DNA microarray and Fluorescence in situ technique? or is the…

A: Difference between FISH and Microarray Technique Fluorescence In Situ Hybridization It is possible…

Q: Explain TWO (2) differences between two commonly used ligases; F. coli DNA ligase and T4 DNA ligase.

A: During DNA cloning process , various restriction enzymes like exonucleases and endonuclease as well…

Q: Briefly explain the rationales of adding Bisacridine A which can affect DNA stability in polymerase…

A: PCR is used to enhance genes associated with patients' DNA genetic disorders. It has practical uses…

Q: You plan to clone exon 11 of the HEXA gene (Section C) into the multiple cloning site (MCS) of the…

A: Answer well explained above

Q: Suppose you have two organisms in a mixture and Organism A is 1,000 times more abundant than…

A: Spread plate technique is generally used to isolate individual colonies from a bacterial culture. It…

Q: What is the attached enzyme in this assay and its corresponding substrate? A. Horseradish peroxidase…

A: INTRODUCTION ELISA Enzyme linked Immunosorbent assay, is a technique used to detect antibodies in…

Q: The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme…

A: In molecular biology procedures, restriction enzymes are especially important for DNA cloning. They…

Q: Parts A-C in the table show the genotypes for two partial diploid strains of the lac operon. Gene…

A: A group of genes called the lac operon are involved in the bacterial metabolism of lactose. *Three…

Q: B. Arrange the following steps in the genetic engineering of corn to acquire pest resistance. to…

A: Genetic engineering is modification in the genetic makeups of the organism in order to get good…

Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…

A: Gel electrophoresis is used to separate molecules of different sizes. It uses charged molecules that…

Q: Hey, I need help with this please: Plasmid pRIT450 is 7.0 kb in length and has single PstI, EcoRI,…

A: Given: A Plasmid pRIT450 of  7.0 kb in length. It has single PstI, EcoRI, and BamHI sites. The…

Q: How does bacteriophage lambda "decide" whether to enter the lytic or lysogenic cycle when it infects…

A: Bacteriophages are viruses that infect bacteria. They are made of nucleic acid enclosed within the…

Q: QUESTION 1 Which of the following would accurately describe the chromosome of a Glow Fish into which…

A: “Since you have asked multiple questions, we will solve the first question for you. If youwant any…

Q: Section Name MAPPING PRACTICE #4 Plasmid pBR 607 is a 2.6 Kb plasmid containing Ampicillin and…

A: Restriction enzymes are one of the important tools of recombinant DNA technology. The name…

Q: 2. Restriction Enzyme Mapping - use any resources to assist you including the hints below. A…

A: Focus only on EcoR1.   Than focus on EcoR1 and HindIII.   Than focus on EcoR1, HindIII and Pst1

Q: Budding yeasts such as S. cerevisiae exhibit telomerase activity throughout their life cycles,…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: why the bacteria synthesize Restriction endonucleases enzymes. What’s the main purpose and how the…

A: Restriction endonucleases (RE) are bacterial enzymes that are used to clip off DNA crosswise at…

Q: You transform tetracycline sensitive competent cells with a plasmid solution (the plasmid encodes…

A: Tetracyclines were discovered in the 1940s and exhibited activity against a wide range of…

Q: MRNA- based therapeutics The following questions: Which research group has initially developed the…

A: Since you have asked multiple questions, as per our honor code we are answering the first three for…

Q: Please explain

A: Lane A:* DNA sample: Human genomic DNA* Enzyme: EcoRI (5' G^AATTC 3')Lane B:* DNA sample:…

Q: Each of the plates was inoculated with E. coli strain 1 that carry a plasmid with Str resistance and…

A: Introduction  A plasmid is a small, circular, double-stranded DNA molecule that exists outside the…

Q: For the DNA sequence shown, indicate the products of its cleavage with the following restriction…

A: In the field of molecular biology and genetic engineering, restriction enzymes, commonly referred to…

Q: Calculate the transformation efficiency of an experiment conducted using 10ul of 0.005 ug/ul…

A: Transformation is the process by which fragment of DNA is taken from the surrounding that causes…

Q: You plate colonies of E. coli with the lac operon an unknown genotype on 1) a medium containing only…

A: The operon is the prokaryotic gene regulatory mechanism that is responsible for the regulation of…

Q: CRISPR. Describe the basic technology (how it works)  and list examples of applications/potential…

A: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a revolutionary gene-editing…

Q: Enzyme(s) used PstI ECORI HincII Band sizes observed (kb) 5.3, 2.7 5.4, 2.6 Smal Xbal ВатHI 4.5, 3.5…

A: Restriction enzymes cut the DNA at a specific sites and into small fragments. This fragments can be…

Q: ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it…

A: First it has to be understood that : 1) the CAP-cAMP complex acts as a glucose sensor and is only…

Q: PCR analysis of transduction colonies. Which one or more than one show successful PCR？why？

A: The polymerase chain reaction, sometimes known as PCR, is a quick and flexible in vitro method for…

Q: Give only typing answer with explanation and conclusion For the genotype shown below, which best…

A: Operon is the prokaryotic gene regulatory system or mechanism that regulates the expression of…

Q: You obtain an E coli mutant with a defect in the pathway that emakes histidine, an amino acid. Tell…

A: Microorganisms such as bacteria are usually cultured in artificial media that contains nutrients…

Q: Who revealed biochemical nature of the transforming principle?how was it done?

A: The genetic material refers to the substance present in mitochondria, cytoplasm, and nucleus. It…

Q: The following image depicts the results of undigested PCR product, and Lane 3 is the restriction…

A: Answer is true .Lane 2 is the undigestive pcr product and the Lane 3 is the digestive product…

Q: Restriction enzyme Restriction site GAATTC CTTAAG EcoRI BamHI GGATCC ССТAGG Hind III АAGCTT TTCGAA…

A: Restriction enzymes are enzymes that cleaves DNA into small fragments at or near specific…