Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Question

RESTRICTION ENZYME MAPPING

Predict the result

PGLO digest Volume to add Tube 1 Tube 2 Mass or Conc. Tube 3 Tube 4 Component Concentration (negative control) (EcoRI, HindlII) (HindllI) required (EcoRI) DNA unknown 1 pg Universal 1X buffer 10X Water EcoRI 0.5 units/ul. 1 unit none Hind III 1 unit none 0.5 units/ul 20 μL 20 μL 20 μL 20 μL ТОTAL
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Transcribed Image Text:PGLO digest Volume to add Tube 1 Tube 2 Mass or Conc. Tube 3 Tube 4 Component Concentration (negative control) (EcoRI, HindlII) (HindllI) required (EcoRI) DNA unknown 1 pg Universal 1X buffer 10X Water EcoRI 0.5 units/ul. 1 unit none Hind III 1 unit none 0.5 units/ul 20 μL 20 μL 20 μL 20 μL ТОTAL
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