RESTRICTION ENZYME MAPPINGPredict the result PGLO digestVolume to addTube 1Tube 2Mass or Conc.Tube 3Tube 4ComponentConcentration(negativecontrol)(EcoRI,HindlII)(HindllI)required(EcoRI)DNAunknown1 pgUniversal1Xbuffer10XWaterEcoRI0.5 units/ul.1 unitnoneHind III1 unitnone0.5 units/ul20 μL20 μL20 μL20 μLТОTAL

Restriction enzymes are those which cut DNA at a specific location called as restriction site.

Answered: RESTRICTION ENZYME MAPPING Predict the…

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RESTRICTION ENZYME MAPPING Predict the result

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

RESTRICTION ENZYME MAPPING

Predict the result

PGLO digest
Volume to add
Tube 1
Tube 2
Mass or Conc.
Tube 3
Tube 4
Component
Concentration
(negative
control)
(EcoRI,
HindlII)
(HindllI)
required
(EcoRI)
DNA
unknown
1 pg
Universal
1X
buffer
10X
Water
EcoRI
0.5 units/ul.
1 unit
none
Hind III
1 unit
none
0.5 units/ul
20 μL
20 μL
20 μL
20 μL
ТОTAL

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