How does that length compare to that of restriction enzymes? Implications?

Q: Q6) Why are ‘sticky ends’ useful biotechnologically? Q7) Why is this enzyme called a restriction…

A: When restriction enzymes cut between two bases on the same strand at one end of the sequence and two…

Q: Plasmid is  3.6kb large. It is digested with an enzyme that has restriction sites at  1,152 and…

A: Plasmids are circular DNA found in bacteria. They are extra chromosomal materials and are highly…

Q: 2- Supposed that you have a DNA sequence sample with 10,000 bp. By using 2 different restriction…

A: The answer is 3.

Q: DNA ligase has the ability to relax supercoiled circular DNA in the presence of AMP but not in its…

A: DNA ligase is the enzyme connecting two DNA strands together. It does so by bonding the phosphate…

Q: Why isn’t cDNA synthetic

A: INTRODUCTION Deoxyribonucleic Acid, or DNA, is a molecule that holds the instructions that an…

Q: Does the addition of a histidine tag affect DNA polymerase activity and or processivity? Give a…

A: DNA polymerase is an important enzyme of dna replication and can add nucleotide triphosphates to 3’…

Q: What is a restriction enzyme? What structure does it recognize?What type of chemical bond does it…

A: Werner Arber, a Swiss microbiologist, winner of 1978 Nobel Prize in Physiology, for his discovery…

Q: How would the results of this activity have been different if the DNA sequence you digested were…

A: Gel electrophoresis is a process in which DNA fragments are separated on the basis of their size.…

Q: What does a deoxy nucleotide do when it gets incorporated any newly synthesize DNA and why? Where do…

A: DNA or deoxyribonucleic acid is the genetic material of most living organisms except for certain…

Q: BamHI Haelll Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 How long is the original DNA molecule?

A: If it is a closed circular DNA, BamH1  cut it into three fragments of length 10kb 5kb 2kb

Q: Why are proteases added while isolating the DNA?

A: Proteases are a group of enzymes which break the proteins into peptides and amino acids.

Q: B) Suppose, you had a linear DNA instead of circular DNA. The double stranded sequence of a…

A: Introduction :- Restriction enzymes, also known as restriction endonucleases, are enzymes that can…

Q: Restriction enzymes in bacterial cytoplasm cut injected bacteriophage DNA wherever certain sequences…

A: The genes are the hereditary unit of an organism. Specific nucleotide sequence of a gene causes the…

Q: QUESTION 24 Answer Assume that a given plasmid vector to be used in a cloning experiment contains…

A: Plasmid is a circular dna molecule used in cloning experiments. This is a small double stranded DNA…

Q: What additive in the agarose gel solution allows the DNA to fluoresce (glow) under UV light? How…

A: Electrophoresis is the migration of charged particles under the influence of an electric current.…

Q: DNA polymerase I, DNA ligase, and topoisomerase I catalyze the formation of phosphodiester bonds.…

A: The DNA replication is a semi-conservative mechanism in which the new complementary strand is…

Q: Hey, I need help with this please: Plasmid pRIT450 is 7.0 kb in length and has single PstI, EcoRI,…

A: Given: A Plasmid pRIT450 of  7.0 kb in length. It has single PstI, EcoRI, and BamHI sites. The…

Q: DNA

A: Answer--

Q: DNA ligase has the ability to relax supercoiled circular DNA in the presence of AMP but not in its…

A: (a). The DNA supercoiling is the manifestation of a structural strain in the DNA by a twisting of…

Q: There are three classes of restriction enzymes; Class I, Class II and Class II. Nevertheless, Class…

A: Restriction endonuclease are the enzyme which make a cut in genetic material that is DNA somewhere…

Q: Why do DNA chips often contain segments derived from cDNA rather than genomic DNA segments?

A: DNA chip used in microarray

Q: In producing genetically engineered human insulin in bacteria, why is it important to use the…

A: Insulin is a natural peptide hormone produced by the beta cells of the pancreas in response to high…

Q: DNA polymerase requires both a template, to be copied, and a primer, which provides a 3′ hydroxyl…

A: DNA polymerase is a main enzyme for DNA replication.. The two main requirements for working of  DNA…

Q: "The mechanism does not recruit a homologous region of DNA during repair". Explain this ?

A: Introduction DNA repair is a set of procedures that a cell uses to detect and repair damage to the…

Q: Can you help with 1a please HELPFUL INFORMATION: When performing classical Sanger or "dideoxy"…

A: Sanger sequencing is done to identify the sequence of DNA. In this process, a particular ddNTP is…

Q: If pET32a(+) is digested simultaneously with BamHI and BclI, 5.1.  How many fragments are created?…

A: pET-32 is a unique sequence outlined for cloning and high-standard peptide sequences expression.

Q: How many fragments and how big each fragment should be after digesting the plasmid with BamHI? How…

A: Digestion of the pUC19 plasmid using the restriction enzymes BamHI and PvuI.1.BamHI Digestion:BamHI…

Q: Which of the following statement is true? * Restriction enzyme binds to non-cognate DNA better than…

A: Restriction enzymes also known as restriction endonucleases are enzymes that recognize and cleave…

Q: The gene you are asked to clone is 30,000 bps in length. When you are choosing a suitable…

A: Introduction  Enzymes are the crucial biomolecules which assists in various biochemical reactions…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.