Q2(a)In 2014, during their iGEM project on biodesalination, students were willing to modifybacteria so that instead of swimming they would float. To do this, they chose to firstknock-out one of the genes for motility (such as MotA) and then insert a gene sequenceto make gas bubbles. They used the restriction enzyme Pstl, which recognizes thesequence 5'-CTGCAG-3' and cuts between A-G. (CTGCA/G). Using the sequencefrom one of the DNA strands of the gene below,5'-TTGATTTTATCGCTGCAGTCGATTATCTGCGCCTGATTATCACTGCAGGCG-3'(fragment modified from the students' plasmid)(v) Provide the double strand sequences resulting from the action of Pstl.(b)(vi)(vii)Specify the name of the ends of the sequences generated and explain theirfunctionality.Describe the last step required to complete the process of deletion started usingPstl(viii) Provide the number of amino-acids deleted through this process and explainwhy such a small deletion could be sufficient to inactivate motility.The team would now like to establish the smallest possible deletion that wouldinactivate the function of the protein. Describe a strategy to carry out this experiment.To check their sequences, the team choses to carry out DNA sequencing themselves,using the Sanger method. Describe the biochemical processes involved in thistechnique in detail.

There are perform a restriction digestion process by restriction endonuclease PstI which recognize…

Answered: Q2 (a) In 2014, during their iGEM…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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