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1. Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.
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- 1. Answer the following questions A. What is the antimicrobial mode of action in the antimicrobial assay? B. Based on the results, interpret the efficacy on the test organism?1. Illustrate in a diagram the identification scheme from gram-positive cocci.2. Discuss the principle involved in the biochemical tests in identifying species of staphylococci.3. Illustrate in a diagram the identification scheme for Streptococci.4. Discuss the principle involved in the biochemical tests in identifying species of Streptococci.1. Use the words presented in below to create a concept map illustrating the relationships among these key terms – refer to “Five I’s” by Cowan and Smith (2018) to be guided. inoculation identification biochemical tests streak plate isolation medium subculturing mixed culture incubation multiplication source of microbes pure culture inspection staining transport medium wire loop 2. What is the importance of employing aseptic techniques? Give an example of a situation in the laboratory that might happen when this method is not practiced.
- 20. Starting with the MacConkey agar and IMVIC tests. Describe how a bacterium can be biochemically identified as Escherichia coli.Ex. 17: Chemotherapeutic Agents Using the Antimicrobial Susceptibility Table on pg 62 in the lab manual, determine whether not the bacteria plated in this Kirby-Bauer test are resistant (R), intermediate (I), or susceptible (S). The bacterial species tested was Escherichia coli (Gram negative). Ciprofloxacin Diameter = 20 Streptomycin Diameter 30 Carbenicillin Diameter 17 + Erythromycin Diameter = 23 Novobiocin Diameter = 0 bacterial growth = disk with antibiotic (see labels) = no growthMethyl red test Does a Phenol red organism have to be methyl red?
- 1. What is the most common sterilization technique used in laboratories? 2. List at least 5 procedures of the aseptic technique and describe its uses. 3. Why is Gram stain one of the most important and widely used stains in bacteriology? 4. Explain the Gram staining technique in chronological order. Indicate the reagent used and the time of usage on each reagent.Name three reasons why the ELISA test is useful in detecting microbesIn the Kirby-Bauer test, which of the following must be consistent? a. concentration of bacteria placed on the plate b. type of medium in the plate c. concentration of antimicrobial drug in the disk d. all of these
- 1. What tests should be undertaken to identify each unkown bacterium in this case study? 2. Discuss atypical results, discrepancies, or other problems that may affect the idetification 3. What is the rationale behind for the choice of tests undertaken to identify the unknown bacterium?1. What are the sources, structural descriptions, and biological functions of gelatin, casein, and albumin? 2. Draw the respective chemical reaction involved in Xanthoproteic test, Millon’s test, Sakaguchi test, Hopkin’s-Cole test, and Sulfur test.Match the confirmation test to the category of bacteria. (each category can be used multiple times) Categories: -Gram Positive - Gram Negative Tests: Match with category A. Citrate test is category.... B. Indole test is category... C. Raffinose test is category... D. Catalase test is category....