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Name three reasons why the ELISA test is useful in detecting microbes
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- What are ELISA assays used for in labs? Give at least three examples.The VP test is a confirmatory test. In what situations would this test be utilized?What other test can be used to confirm the results obtained for a TEV infected tomato in an ELISA test? Name and very briefly describe one other test. Give the reason for your choice.
- Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the microtiter wells? Why is it important to have a positive control?1. You are asked to make 150 ml of 2% agarose using a well-known buffer. How many grams will be used? a) 0.03 g b) 0.3 g c) 3 g d) 30 g 2. Place the following reactants in their proper order for the indirect ELISA test 1 = enzyme-linked antibody 2 = known antigen 3 = patient serum 4 = substratein an indirect elisa procedure what enzyme is used?
- Counterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blottingIn the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different?b) What is “coating buffer” for an ELISA? Does the buffer molecule usedmake sense based on the desired pH?In an ELISA procedure the samples are incubated and the ELISA plate iscovered with parafilm and placed in a humidified chamber to preventevaporation of the small liquid volumes in the wells.c) How would your results change if you did not incubate in a humidifiedchamber?In relation to ELISA technique, fill in the missing information in the figure below: 1- 2- 3- 4-
- a) did the positive and negative controls work correctly in this ELISA? Explain. B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?What other test can be used to confirm the results obtained in this ELISA test? Name and very briefly describe one other test. Give the reason for your choice.What are the differences between “O” and “H” antigen? Differentiate Widal from Weil-Felix test. The strains of Proteus vulgaris are used in Weil-felix reaction. Explain why.