Phage DNA- he Phage Infects bactertal coll Donor coll- (Infacted by a virus) that Is hes. Host DNA Is digostad Into fragmonts, and phago DNA and proteins are made. his Phagas assemble; occasionally a phago carrles a fragment of the host cell chromosome. In this casa, It carrles the hts gane Transduction in bacteria. Genes+Traits During transduction, a phage carries a segment of bacterial DNA from a donor to a recipient cell. In this case, the phage carried a segment of DNA with the his+ gene and transferred this gene to a recipient cell that was originally his- (unable to synthesize histidine). Following transduction, the recipient cell became his+, and thus able to synthesize histidine. Transducing phage with host DNA Transducing phage Injects Its DNA into a recipient cell that Racombination his- Raciplent coll- The transduced DNA Is recombined Into the chromosome of the reciplent cell. his Recombinant bacterium The racombinant bacterium's genotype has changed from his- to his".

Biology: The Unity and Diversity of Life (MindTap Course List)
15th Edition
ISBN:9781337408332
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter20: Viruses, Bacteria, And Archaea
Section: Chapter Questions
Problem 3DAA: Bacteriophage-Inspired Antibiotics Although bacteriophages have been infecting bacteria for billions...
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In a P1 transduction experiment, the P1 lysate contains phages that
carry pieces of the host chromosomal DNA, but the lysate also contains
broken pieces of chromosomal DNA. If a P1 lysate is used to transfer chromosomal DNA to another bacterium, how could you show experimentally that the recombinant bacterium has been transduced (i.e., has taken up a P1 phage with a piece of chromosomal DNA inside) versus transformed (i.e., has taken up a piece of chromosomal DNA that is not within a P1 phage coat)?

Phage DNA-
he
Phage Infects
bactertal coll
Donor coll-
(Infacted
by a virus)
that Is hes.
Host DNA Is digostad
Into fragmonts, and phago
DNA and proteins are
made.
his
Phagas assemble;
occasionally a phago
carrles a fragment of
the host cell chromosome.
In this casa, It carrles the
hts gane
Transduction in bacteria. Genes+Traits During transduction,
a phage carries a segment of bacterial DNA from a donor to
a recipient cell. In this case, the phage carried a segment
of DNA with the his+ gene and transferred this gene to a
recipient cell that was originally his- (unable to synthesize
histidine). Following transduction, the recipient cell
became his+, and thus able to synthesize histidine.
Transducing
phage with
host DNA
Transducing phage
Injects Its DNA into
a recipient cell that
Racombination
his-
Raciplent coll-
The transduced DNA
Is recombined Into the
chromosome of the
reciplent cell.
his
Recombinant
bacterium
The racombinant bacterium's genotype has
changed from his- to his".
Transcribed Image Text:Phage DNA- he Phage Infects bactertal coll Donor coll- (Infacted by a virus) that Is hes. Host DNA Is digostad Into fragmonts, and phago DNA and proteins are made. his Phagas assemble; occasionally a phago carrles a fragment of the host cell chromosome. In this casa, It carrles the hts gane Transduction in bacteria. Genes+Traits During transduction, a phage carries a segment of bacterial DNA from a donor to a recipient cell. In this case, the phage carried a segment of DNA with the his+ gene and transferred this gene to a recipient cell that was originally his- (unable to synthesize histidine). Following transduction, the recipient cell became his+, and thus able to synthesize histidine. Transducing phage with host DNA Transducing phage Injects Its DNA into a recipient cell that Racombination his- Raciplent coll- The transduced DNA Is recombined Into the chromosome of the reciplent cell. his Recombinant bacterium The racombinant bacterium's genotype has changed from his- to his".
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