Penicillin heralded the dawn of the antibiotic age when it was discovered in 1928 and is now the most widely used antibiotic in the world. A membrane electrode was developed for the quantitative analysis of penicillin. The table above shows the data which were collected using a set of penicillin standards against saturated calomel electrode. An unknown sample produced a potential of 142 mV.  A) Which served as the reference electrode? B) What is the molar concentration of penicillin in the sample?

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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Penicillin heralded the dawn of the antibiotic age when it was discovered in 1928 and is now the most widely used antibiotic in the world. A membrane electrode was developed for the quantitative analysis of penicillin. The table above shows the data which were collected using a set of penicillin standards against saturated calomel electrode. An unknown sample produced a potential of 142 mV. 

A) Which served as the reference electrode?

B) What is the molar concentration of penicillin in the sample?

[penicillin] (M) potential (mV)
1.0 × 10-2
220
2.0 × 10-3
204
1.0 x 10-3
190
2.0 × 104
153
1.0 × 104
135
1.0 × 10-5
96
1.0 × 10-6
80
Transcribed Image Text:[penicillin] (M) potential (mV) 1.0 × 10-2 220 2.0 × 10-3 204 1.0 x 10-3 190 2.0 × 104 153 1.0 × 104 135 1.0 × 10-5 96 1.0 × 10-6 80
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