If you need 2.5 mg/liter, 2,4-D in 1 liter of medium and the stock solution of 2,4-D contains 10 mg/100 ml, how much stock solution do you need? why BAP is preferred over other cytokinin during commercial plant tissue culture
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If you need 2.5 mg/liter, 2,4-D in 1 liter of medium and the stock solution of 2,4-D contains 10 mg/100 ml, how much stock solution do you need?
why BAP is preferred over other cytokinin during commercial plant tissue culture
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- Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?A culture of S. cerevisea has an overnight OD of 4.5 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 4.5 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ulA vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
- Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.If the volume of a Staphylococcus aureus cell is estimated at 0.5 μm3, how many cells could be accommodated, in principle, in 5 mL of saturated culture? (1 mL = 1 cm3). Show your calculations.If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperation
- the optical density of your cell culture is 0.92. How much of this culture should you add to 500 mL of LB to obtain a starting optical density of 0.1?You have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose?We’re back in the lab having fun! Our current experiment calls for us to treat our cells with THC (yeet!, delta 8 from hemp of course lol) and the final concentration of 15 µM once it has been added to the cells. We need to treat 10 mL of cells, and we don't want our treatment volume to be more than 10 µL per 1 mL of cells. What concentration should we make our stock solution? (I submitted this question before and got an answer of 150 µM and that was incorrect)
- Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.A mixture of dipeptides consisting of Lys-Gly, Thr-His, Ser-Leu, Glu-Gln was applied to column packed with cation-exchange resin at pH 6.0. Which of these dipeptides would be eluted from the column first? O Lys-Gly first O Thr-His first O Ser-Leu first O Glu-Gln first OA mixture of Thr-His and Ser-Leu firstIn an experiment, you are treating cells in culture with drug X that was dissolved in The stock of drug X is 10 mM. The final treatment concentration needs to be 10 nM. You add the stock drug directly into 10 mL of cell culture media to reach the treatment concentration. How many ul of DMSO do you need to add to 10 mL of cell culture media for the vehicle control cells? 10 mL cell culture media with vehicle 10 mL cell culture media with 10 nM drug X Vehicle treated cells Drug treatment cells Drug X stock 10 mM