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- Which electron or light microscopy technique would you use for the following? (also explain) For visualizing the fine structure of cellular organelles and cell architecture in the cell interior using very thin cross-sections made in Epon. To obtain sequential images of a macromolecule in order to study its activity in real-time. To obtain an extremely clear three-dimensional image of the nucleus of a single cell. What is the magnification of the image in a microscope using a 20x ocular and a 100x objective?The following fluorescence microscopy shows several cells. What part of the cell is labelled by the fluorescence here? b The Cytosol The Endoplasmic Reticulum The nucleolus The nucleus The actin cytoskeletonPlease match the junction type with the cytoskeletal component it is associated with Desmosome Tight junction Adherens junction Focal adhesion Hemidesmosome ✓ [Choose ] microtubule none intermediate filament actin [Choose ] [Choose ] [Choose ]
- Discuss the structure and function of the Golgi apparatus.Put the following structures in order according to the pathway of a secreted protein: a. plasma membrane b. Golgi bodies c. endoplasmic reticulum d. post-Golgi vesiclesDescribe the following as seen under the compound microscope. Endoplasmic reticulum Ribosome Centrosome
- ER lumen: C- terminus or N- terminus Golgi apparatus- C- terminius or N- terminus transport vesicles lumen- C-terminus or N-terminusStructural and functional features of the lysosomes include I. internal pH is higher than the cytosol to provide an acidic pH for the enzymes II. single membrane with a hydrogen ion pump to maintain an acidic pH III. filled with hydrolytic enzymes that are glycosylated IV. its contents are delivered by clathrin-coated vesicles from the TGN II, III, IV only I, II, III only I, II, III, IV I, II, IV onlygive the comparison between lysosomes, vacuoles, and peroxisomes. lysosomes vacuoles peroxisomes
- The properties of integral membrane proteins can be studied by 1)Atomic force microscopy 2)Cryo-sectioning and electron microscopy 3)Freeze-fracture technique and electron microscopy 4)All of the aboveMagnifying power of eyepiece Which cell organelle is involved in apoptosis * ER Mitochondria Golgi LysosomeFrom the options shown what proteins types/groups would not be present in vesicles budding from the trans-Golgi network? O proteins destined for the plasma membrane O proteins destined for the peroxisomal matrix O proteins destined for the endosome O proteins destined for the lysosome, O proteins secreted by exocytosis
