When preparing cells for karyotyping, in which of the following solutions should the cells be immersed? A.-Isotonic B.-Hypertonic C.-Melted Sugar D.-Hypotonic E.- Saline at human body temperature
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When preparing cells for karyotyping, in which of the following solutions should the cells be immersed?
A.-Isotonic
B.-Hypertonic
C.-Melted Sugar
D.-Hypotonic
E.- Saline at human body temperature
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- Which of the following is TRUE about immunofluorescence microscopy where we localize proteins using fluorescently tagged antibodies? Pick the best answer: We can use it to resolve 2 points that are 10 nanometers apart. The emission wavelength of the fluorophore is typically shorter than the excitation wavelength. The resolution can be further improved with increased magnification. All else being equal, a fluorophore that emits red light will give better resolution than a fluorophore that emits green light. Based on what we discussed in class, we can use the technique on live samples therefore enabling us to see the dynamic movement of cytosolic proteins in living cells. None of the aboveThe cell is usually placed after the monochromator, except in the case of using a multichannel detector. Why?Assume that you are starting with a sample containing 6 billion active cells. Create a dilution plan to reduce this count to a range of 30-300 colonies on a plate. The powdered sample weighs 500 mg (0.5 g). You can use up to 4 plates – you should plate different dilutions and/or volumes of solution. Start your first dilution by adding 10 mL diluent to 0.5 g powder, which is a 1/20 dilution. Hint: Rearranging the “cfu/ml” formula from our dilutions lab can help you solve this. Choose a target cfu count, and your volume plated should be under 0.5 ml.
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- Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn vWhen doing a routine differential count for a CBC how many cells are counted? a) 50 b 75 c 100 d 125 e 150Which two fluorescence-based methods allow localization of proteins in vivo? Group of answer choices FRET and FRAP FRET and FLIP FRET and immunofluorescence Conjugation of fluorescent protein to protein of interest and immunofluorescence
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