Q: Define electrophoresis
A: Gel electrophoresis is a molecular biology technique used in laboratories.
Q: identiry at least four difere gel electrophoresis.
A: Four factors that will impact the how far molecules will move during gel electrophoresis 1. Sample…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Explain the process behind coulometry.
A: Coulometry is a method which is based on the measurement of the quantity of electricity which is…
Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…
A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…
Q: Describe the Gram staining procedure
A: Gram staining is used to differentiate between Gram positive and Gram negative bacteria. These…
Q: Create a flow chart on how gel electrophoresis is being conducted
A: Gel electrophoresis is a molecular technique that helps us to separate a mixture of DNA sample based…
Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…
A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…
Q: Transillumination is used clinically for the detection of:
A: Transillumination is an examination that is done with the help of a strong light shining on a…
Q: Why is the culture medium referred to as a highly enriched laboratory soil?
A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…
Q: Discuss about the reagents used for agarose gel electrophoresis
A: Gel electrophoresis is used to separate nucleic acid.
Q: Define gel electrophoresis?
A: DNA is vital for all living beings. It is important for inheritance, coding for proteins, and the…
Q: describe the agar-overlay technique
A: Biological techniques are processes or methods for trying to study living things. They include…
Q: Identify the following on the gel electrophoresis 2-leg ladder 3 Kb 1022 bp 1022 bp 880 bp 765 hp…
A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: Why is it important to use a sterilized loop between streaks when preparinga streakplate?
A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.
A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…
Q: What is the difference between streak plate method and spread plate technique?
A: Answer of the question given below...
Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a…
A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…
Q: What are two types of compounds that make up the resin for column chromatography?
A: Column chromatography is a technique used for the separation or purification of biomolecules such as…
Q: Draw and explain the process of electrophoresis utilized in decalcification.
A: Decalcification :- As name suggests, it is removal of calcium salts,minerals and calcium tissues…
Q: Describe the agar-overlay technique
A: Biological techniques are methods or processes used to study living things. Methods, approaches,…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Give three situations where the negative staining procedure would be used.
A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…
Q: Explain the function of stacking gel, separating gel, and Coomassie blue stain in the…
A: Answer : Stacking Gel : The purpose of the stacking gel packing is to combine all the samples of the…
Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…
A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…
Q: Why differential centrifugation can cause contamination in pellet?
A: Differential centrifugation is a typical process used in microbiology and cytology to separate…
Q: List the reagents of the Gram stain technique in order and their general role in the staining…
A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…
Q: What is meant by the term “enrichment culture”?
A: The culture medium or growth medium is the specific medium, which contains all essential nutrients,…
Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE…
A: Indole test is a biochemical test for distinguishing between Enterobacter aerogenes and Escherichia…
Q: List four reasons for the development of pulsed field gel electrophoresis
A: Electrophoresis is a general term that describes the migration and separation of charged particles…
Q: Distinguish batch culture and continuous culture
A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…
Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…
A: Fluorescence quenching is a method that diminishes the fluorescence force of an sample. A number of…
Q: List the step-by-step procedure of Gram staining.
A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…
A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…
Q: Explain the conclussion about gel electrophoresis using 3 different food dyes (yellow, blue and…
A: The method used to separate mixtures of DNA, RNA, or proteins according to their size is known as…
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: Write down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin…
A: Polychromatic solution of Methyl Blue and Eosin Y stains (modified Mann's solution) is used for…
Q: When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies…
A: Dilution is the process of decreasing the concentration of the solution. The cells count decreases…
Q: What are the advantages and disadvantages of using the Slide Culture technique?
A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…
Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…
A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…
Q: ative gel electrophoresis
A: Separation techniques are critical for identifying and isolating biomolecules for further biological…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: Explain the methods and materials in gel electrophoresis using 3 different food dyes (yellow, blue…
A: Gel electrophoresis is an analytical technique used in the lab to separate mixtures of DNA, RNA…
Q: What are the different manufacturing methods of Suppositories dosage form? Explain heat molding…
A: Suppositories are the solid medication that delivers drug in rectum ,vagina and or urethera. Most…
Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?
A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: In RPR test, Why is it necessary to rotate the slide/card?
A: RPR stands for Rapid Plasma Reagin. It is done to detect syphilis.
Name the substance used as a medium in gel electrophoresis.
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- What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.What are the different applications of Gel Electrophoresis? explain each applicationsWhen working with the gel electrophoresis chamber, what must we keep in mind?