What are the different manufacturing methods of Suppositories dosage form? Explain heat molding process using flow chart.  Please explain at your own words.

You are tasked to isolate a certain species of bacteria from a mixed culture to further characterize and identify the strain. Which technique/s is Best to perform? How shall the procedure be carried out? Explain.

Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?

The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals", describes which technique?

What is the principle behind invertase test?

Explain the procedure (Simply) for collection of specific protein from the plant by extraction method. Give it as in order use bulletin.

Discuss and explain the results of this graph. Graph shows results of WST-1 assay. T0 ( time zero) shows assay performed in order to obtain an absorbance at the time of test agent added in order to determine 1. how much cells have grown over the incubation period 2. Growth inhibition by test agent 3. If test agent caused cytotoxicity

Calculation for a neutral red assay please include steps since it is essential for the report and it is marked.

You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]

Draw a 4-inch circle. Draw out the streaking pattern used to isolate single colonies on a plate.