what are the reagents for the four major steps of the gram stain procedure

Explain the principle of Gram staining. (You do not need to describe the procedure step by step).

Differentiate the “hot method” and “cold method” of acid-fast staining

For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?

What are the 4 major steps in Gram Staining and what is the role of each reagent?

What are the materials and methods to achieve identification code (not the actual identification code) and the method to achieve the unknown identity since the "unknown organism" is identified using a numerical code from the bioMerieux catalog/APIweb that lists each reference species with its 7 digit code. Gram stain of the isolate to be identified, the oxidase test , the API 20E strip used and the reagents added .

Describe the color and appearance of a Gram positive and Gram negative bacteria as it is treated at every step of the Gram staining procedure

What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?

EMB agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. It is particularly effective at determining if E. coli is present in a specimen. Define what type(s) of media EMB agar is, using as many terms in the answer list as are applicable.

What is the purpose of iodine in the gram staining process? Explain.