Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: what is the procedure and materials you need to stain Staphylococcus aureus?
A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium hat belongs to the Firmicutes and…
Q: what is the importance of getting retention factor (rf) value in chromotography tools?
A: Rf also known as retention factor is used in thin layer chromatography (TLC) and high performance…
Q: explain why it is not good to break sterile field when inserting a foley catheter
A: Inserting the indwelling catheter is an invasive procedure. Sterile technique should follow while…
Q: What are the underlying physical principles of paper chromatography? What is spectrophotometry, the…
A: Analysis of different compounds quantitatively and qualitatively is an important aspect of research…
Q: The ___________ color test reagent turns orange-brownin the presence of amphetamines.
A: A biochemical substance that induces physiological responses, when introduced into the body, is the…
Q: Why might Gamma irradiation be considered better than using UV radiation?
A: It is required to provide reason that Gamma irradiation is considered better than using UV radiation…
Q: Why is there a need to use a staining agent in observing the specimen?
A: Staining agent: The things that owes-out stains were recognized as staining agent,…
Q: How are clinical chemistry test results used to confirm or rule out a presumptive diagnosis? Why is…
A: Any medical test's primary goal is to assess if a patient does or does not have a specific disorder.
Q: What pathogenic agent undergoes the Sigmoidoscopy Material procedure?
A: The sigmaidoscopy procedure test uses a thin flexible tube with a camera and light on one end. The…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…
A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…
Q: Which objective is referred to as the "scanning objective"? 4x 10x 20x 100x 400x
A: The least powerful lens is called the scanning objective lens and is typically a 4X objective. A…
Q: Explain the usage of photoactivated localization microscopy technique.
A: Photoactivated localization microscopy abbreviated as PALM is a microscopy technique that was…
Q: Application of differential staining technique.
A: Differential staining is a staining technique that employs the application of several different…
Q: Preservatives are added to the following pharmaceutical products EXCEPT Select one: Ophthalmic…
A: Any substances that are added to the foods and pharmaceutical products to prolong its shelf life…
Q: Why is the accuracy of the calibration curve so critical?
A: Calibration curves are the graphs showing how analytical or instrumental response changes with the…
Q: Why is UV light used in Column chromatography experiment ?
A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…
Q: g technique, and what is the target organisms and the purpose of its application? LONG ANSWER…
A: The bacterial staining can be simple as well as differentiated. It is studied under the domain of…
Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.
A: Mass Spectrometry is an analytical method. It is used to quantify known materials to identify…
Q: What are the possible sources of contamination that may result during the streak plate procedure?
A: The streak plate procedure is used to isolate microbial colonies in pure form. During the procedure,…
Q: Why is it necessary to use immersion oil when the 100X objective is used'
A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…
Q: Discuss sabin feldman dye test.
A: A Sabin–Feldman dye test is a serologic test for toxoplasmosis diagnosis. Toxoplasma trophozoites…
Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…
A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.
Q: Discuss the significance of the following processes and what constituent is being determined: a.…
A: Borntrager’s Test: This is a quantitative chemical test for the glycosides in any given sample, this…
Q: Give three situations where the negative staining procedure would be used.
A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…
Q: What is the importance of performing the Modified Allen Test? Give at least three.
A: Modified Allen test: This test is done to assess the integrity of the ulnar artery, To perform this…
Q: Sources of error and precautions when doing the creatinine test using the Jaffe Method
A: Creatinine is the degraded product of creatine and phosphocreatine, which is used to test the renal…
Q: What color tube is used for creatinine test?
A: Introduction:- Creatinine test is done to assess the situation of the kidney. Creatinine values…
Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…
A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…
Q: Why are specimens for bilirubin testing protected from light? Discuss handling procedures for…
A: Bilirubin is a yellow pigment that is present in bile. It is formed as a result of breakdown of red…
Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?
A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…
Q: A photo-multiplier tube converts light into x-rays. True False
A: 1. ANS -TRUE, PHOTON MULTIPLE TUBE ( PMT) CONVERT LIGHT TO X RAY . A photomultiplier tube (PMT)…
Q: True or False: Ultrathin sections are used for light microscopy but are to thin to be stable under…
A: Microscopes are used to visualize objects that are not visible to the naked eye. In microbiology,…
Q: The most commonly employed chemical test to determine the presence of blood in stain found at the…
A: Answer is option E"Teichman's test". this test is also called by names haematin crystal acid test or…
Q: What tests are included in a CBC? Give the normal value,and name a disorder that would cause an…
A: Complete blood test It is the test to perform to evaluate various parameters of blood cells. Purpose…
Q: Why is it incorrect to say that the GFP is a “staining” method?
A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…
Q: If, when using 10X ocular and a 20X objective, the field size is 2 mm. The approximate field size…
A: Ocular magnification - This is the magnification that can be viewed by the ocular lenses, the lenses…
Q: What advantage does the oil immersion objective have over the low power objective when doing…
A: Introduction:- Bacteria are prokaryotic and very small sized organism's . They can not be visualised…
Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?
A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…
Q: Write down the materials, reagents, Principle, procedure and results for Ziehl-Neelsen stain
A: It is a differential staining technique which was first developed by Ziehl and later on modified by…
Q: The Duquenois-Levine test is a valuable color test for
A: We can say that, In a forensic chemical analysis of an unknown material, the analytical method is to…
Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…
A:
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: In RPR test, Why is it necessary to rotate the slide/card?
A: RPR stands for Rapid Plasma Reagin. It is done to detect syphilis.
Q: Why rapid tests are not the gold standard in Covid19 detection. Explain.
A: Diagnosis of the Sar-Cov-2 virus is usually done by means of isolating samples collected from the…
Q: The Duquenois–Levine test is a valuable color test for___________.
A: In a forensic chemical analysis of an unknown material, the analytical method is to narrow down the…
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- What are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?One laboratory method to detect for COVID-19 infection is via real time RT-PCR. The process starts with nasopharyngeal or oropharyngeal swabbing to get a sample. Explain concisely how this technique generally works and how it can be used for testing.