Ms. Michi is a researcher in a renowned pharmaceutical company based in Singapore. One of the cell lines in the lab is suspected to be contaminated. She is culturing NIH 3T3 mouse embryo fibroblast cell line (NIH/3T3 (ATCC® CRL-1658TM). Figure 1 below illustrates her observation under the inverted phase contrast microscope. a) b) c) d) e) Figure 1 (Routray et al., 2016) What type of contamination is her laboratory facing? Explain your answer. Give FOUR (4) suggestions to Michi in order to prevent such contamination in future Besides the contamination stated in Question 2 a), name FIVE (5) other contaminations that Michi should be aware of. During a routine check, Michi's superior requests her to characterize cell lines available in the storage to determine their authenticity. Describe TWO (2) effective cell line characterization methods that she can utilize for this purpose. The pharmaceutical company wants to improve its toxicity testing by replacing monolayer culture with spheroid culture system. Describe the advantages of spheroid culture.
Ms. Michi is a researcher in a renowned pharmaceutical company based in Singapore. One of the cell lines in the lab is suspected to be contaminated. She is culturing NIH 3T3 mouse embryo fibroblast cell line (NIH/3T3 (ATCC® CRL-1658TM). Figure 1 below illustrates her observation under the inverted phase contrast microscope. a) b) c) d) e) Figure 1 (Routray et al., 2016) What type of contamination is her laboratory facing? Explain your answer. Give FOUR (4) suggestions to Michi in order to prevent such contamination in future Besides the contamination stated in Question 2 a), name FIVE (5) other contaminations that Michi should be aware of. During a routine check, Michi's superior requests her to characterize cell lines available in the storage to determine their authenticity. Describe TWO (2) effective cell line characterization methods that she can utilize for this purpose. The pharmaceutical company wants to improve its toxicity testing by replacing monolayer culture with spheroid culture system. Describe the advantages of spheroid culture.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Ms. Michi is a researcher in a renowned pharmaceutical company based in Singapore. One of the cell
lines in the lab is suspected to be contaminated. She is culturing NIH 3T3 mouse embryo fibroblast
cell line (NIH/3T3 (ATCC® CRL-1658TM). Figure 1 below illustrates her observation under the
inverted phase contrast microscope.
a)
b)
c)
d)
e)
Figure 1 (Routray et al., 2016)
What type of contamination is her laboratory facing? Explain your answer.
Give FOUR (4) suggestions to Michi in order to prevent such contamination in future
Besides the contamination stated in Question 2 a), name FIVE (5) other contaminations that
Michi should be aware of.
During a routine check, Michi's superior requests her to characterize cell lines available in
the storage to determine their authenticity. Describe TWO (2) effective cell line
characterization methods that she can utilize for this purpose.
The pharmaceutical company wants to improve its toxicity testing by replacing monolayer
culture with spheroid culture system. Describe the advantages of spheroid culture.
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