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Look at the synthesis of citryl CoA by citrate synthase in the figure.  Which of the 4 general catalytic mechanisms are present? Catalysis by approximation   Covalent Catalysis   General Acid-Base Catalysis   Metal Ion Catalysis

Look at the synthesis of citryl CoA by citrate synthase in the figure.  Which of the 4 general catalytic mechanisms are present? Catalysis by approximation   Covalent Catalysis   General Acid-Base Catalysis   Metal Ion Catalysis

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Look at the synthesis of citryl CoA by citrate synthase in the figure.  Which of the 4 general catalytic mechanisms are present?

Catalysis by approximation
 
Covalent Catalysis
 
General Acid-Base Catalysis
 
Metal Ion Catalysis
**Transcription for Educational Website:** ### Diagram Explanation: Enzymatic Reaction Pathway The diagram illustrates the enzymatic conversion process involving oxaloacetate and acetyl CoA to form a citryl CoA complex through an enol intermediate. The process is shown in three main stages, each represented by a molecular structure within an active site, highlighted in yellow. #### 1. Substrate Complex - **Components**: Oxaloacetate and Acetyl CoA - **Key Interactions**: - **Histidine 320** and **Aspartate 375**: These amino acid residues are positioned to facilitate the interaction. - **Histidine 274**: Interacts with the substrate complex, contributing to stabilization. - **Macromolecules**: - **Oxaloacetate**: Presented with carboxylate groups in blue. - **Acetyl CoA**: Highlighted in magenta. #### 2. Enol Intermediate - **Description**: Transition state between substrates and product. - **Key Reactions**: - Step **①** and **②** denote shifts in molecular structure aiding the progression. - **③** indicates the formation of the enol intermediate through enzyme-assisted protonations and deprotonations. #### 3. Citryl CoA Complex - **Resulting Product**: Formation of Citryl CoA. - **Structure**: - Combines previous molecular structures with key interactions via the amino acids. - **Final Arrangement**: Stabilized by the active site residues, indicating successful transformation from reactants to a complex product. The diagram provides a step-by-step visual understanding of the enzyme-catalyzed reaction, emphasizing the role of specific amino acids and the structural changes leading to product formation.
Transcribed Image Text:**Transcription for Educational Website:** ### Diagram Explanation: Enzymatic Reaction Pathway The diagram illustrates the enzymatic conversion process involving oxaloacetate and acetyl CoA to form a citryl CoA complex through an enol intermediate. The process is shown in three main stages, each represented by a molecular structure within an active site, highlighted in yellow. #### 1. Substrate Complex - **Components**: Oxaloacetate and Acetyl CoA - **Key Interactions**: - **Histidine 320** and **Aspartate 375**: These amino acid residues are positioned to facilitate the interaction. - **Histidine 274**: Interacts with the substrate complex, contributing to stabilization. - **Macromolecules**: - **Oxaloacetate**: Presented with carboxylate groups in blue. - **Acetyl CoA**: Highlighted in magenta. #### 2. Enol Intermediate - **Description**: Transition state between substrates and product. - **Key Reactions**: - Step **①** and **②** denote shifts in molecular structure aiding the progression. - **③** indicates the formation of the enol intermediate through enzyme-assisted protonations and deprotonations. #### 3. Citryl CoA Complex - **Resulting Product**: Formation of Citryl CoA. - **Structure**: - Combines previous molecular structures with key interactions via the amino acids. - **Final Arrangement**: Stabilized by the active site residues, indicating successful transformation from reactants to a complex product. The diagram provides a step-by-step visual understanding of the enzyme-catalyzed reaction, emphasizing the role of specific amino acids and the structural changes leading to product formation.
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