Non-denaturing Denaturing Mwt Stas, KD 272 132 - 95 66 - 45 14 11
The enzyme pyruvate carboxylase is discovered in a bacterium that was thought not to contain it; in this case study, you’ll see how researchers study and characterize the enzyme, and, ultimately, show how it fits into a
Following purification of the PYC enzyme by avidin-Sepharose affinity chromatography, the investigators carried out several experiments to characterize the enzyme.
First, they ran samples of the enzyme on denaturing and non-denaturing gels; the results are shown in the figure to the right. In addition, they ran the protein through a calibrated gel filtration column, the results of which indicated that the PYC enzyme had a molecular weight of 540 kiloDaltons. In the figure, the rightmost column are where molecular weight standards of various sizes would occur if they’d been on the gels.
Part A: There are four identical subunits in the PYC enzyme. And there are not four domains in the denaturing gel. Based on the image provided attached, how many chains does the denaturing gel suggest each subunit has?
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