Label the following parts of the CRISPR-Cas 9 system. C A B Target DNA strand PAM sequence Cas9 protein Single-guide RNA

In Northern blot analysis, mRNA samples from tissues are bound to a labeled DNA probe that is complementary to the mRNA, and run on a gel to be visualized. The protein tropomyosin is known to be present in both brain and liver.  When brain and liver tissue were assayed for the presence of tropomyosin mature mRNA, bands of two different sizes were seen. Tropomyosin gene diagram (3000 bp total): Shown in attatched image If the band on the Northern blot for mRNA isolated from liver tissue is 2580 bp, whereas from brain tissue the band is 2250 bp, what is most likely? a)The two mRNAs are made from different tropomyosin DNA sequences. b)Exon 2 is alternatively spliced out of the brain mRNA. c)Introns 1 and 2 are spliced out of the brain transcript but not the liver transcript. d)Exons 1 and 3 are spliced out of the brain transcript but not the liver transcript. e)Exon 2 is alternatively spliced out of the liver mRNA.

Which of the following can be used to silence specific genes in a laboratory setting? Histone aceylation Gene duplication RNA Interference Alternative Splicing

The new vaccines use messenger RNA (mRNA) in order to trigger the body into mounting an immune response against the viral particles of SARS-CoV-2.   SARS-CoV-2 is the name of a type of coronavirus that causes the disease, Covid-19.   As you know, mRNA carries the instructions to build proteins.   What specific proteins does the mRNA in the adult Moderna vaccine code for?

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Gene expression can be disrupted by techniques such as homologous recombination and RNA interference. What is the functional difference between these two methods in terms of the ultimate effect on gene expression? Why might homologous recombination or an alternative genome-editing method (e.g., CRISPR/Cas9) be preferred over RNA interference?

Line A below shows the peptide synthesized after transcription and translation of a piece of DNA.  Line B shows the peptide synthesized after a mutation in this piece of DNA. proline- arginine - aspartic acid - lysine - glycine proline - glutamine - aspartic acid - lysine — glycine The type of change in the DNA most likely to be responsible for the difference between the peptides is:   a. A frame-shift mutation d. A base deletion b. A base insertion e. A silent mutation f. A base substitution