For a coding gene, purifying (negative) selection will result in more synonymous (silent) site changes when compared to the nonsynonymous (replacement) site changes. 1)True 2)False

Pinpointing a disease gene requires a combination of approaches. Mutations in the XIAP gene are known to cause a serious condition called X-linked lymphoproliferative disease (XLPD).   In the case  of XLPD, in which the blood contains too many lymphocytes (white blood cells of the immune system), crowding out the oxygen-carrying red blood cells and damaging the liver.  When compairing the XIAP amino acid sequence of one affected patient to other human and animal standard RefSeq, the patient has an amino acid substitution at position 203, from cystenine (C) to tyrosing (Y).  This evolutionary conservation suggests  Group of answer choices that the standard RefSeq does not provide enough information to determine a possible cause of  XIAP function. mutations in the XIAP gene and XLPD are not associated.   that the mutation in the affected patient has no impact on XIAP function. that the mutation in the affected patient might alter XIAP function.

You are employed in a gene therapy laboratory to test the effectiveness of a vector for correcting the DF508 –CFTR mutation.  The vector uses CRISPR –CAS9 genome editing to correct the mutated gene in situ (CFTR-CRISPR). An Ussing chamber experiment was performed using nasal airway cells obtained from a patient with the DF508 CFTR mutation which were transformed with either a control vector consisting of an inert CRISPR-CAS-9 construct (CONTROL) or the active, gene correcting CRISPR CAS-9 construct (CFTR-CRISPR).  The following transepithelial voltage values were obtained for the drug regimen shown under open circuit and current clamp (injected current of 10mA) conditions: CONTROL   Baseline Amiloride Forskolin Inh172 Vte (mV) -78 -2 -4 -3 DVRt (mV) 8 16 7.5 13 R (W.cm-2) 800 1600 750 1300 I (mA.cm-2) 98 1 5 2 CFTR-CRISPR Vte (mV) -38 -6 -23 -9 DVRt (mV) 9 27 6 11 R (W.cm-2) 900 2700 600…

i) Suppose we want to insert the GFP sequence after the promoter of a gene X to create a fusion protein. Please describe the strategy that you will follow to edit this gene with CRISPR-Cas9 technology, in order to visualize the expression of gene X in live cells. (ii) Please explain how a knockout mouse for a gene X can be produced with CRISPR-Cas9 technology

13) The pLacZ cloning vector has an MCS with 10 different palindromes, that is, where you can clone using 10 different enzymes. How many different combinations of enzymes could be used to clone? (This is a measure of the versatility of the vector) a) 2^10 b. 4^3 c. 10^2 d. 3^4 14)One of the first vaccines achieved thanks to the tissue culture technique was that of influenza, True or False? 15) In the context of biotechnology, what does the term clone mean? a) Synonym of Dolly, identical copies of a multicellular organism b. Many copies of some cellular organelle. c. Production of drugs and medicines for diseases, but under the control of ethical standards d. Chimeric molecules (vector and insert), inside a bacteria replicating to form a colony e. Identical copies of proteins from recombinant DNA

Using COVID-19 ICD-10 Diagnosis Code U07.1, explain your experience with entering this code in a coding website: Are there any listed guidelines in using this code (see ICD-10 guidelines in book), if so, what are they? What does your research tell you about the benefits and drawbacks of using the Codify software?

Knock out mice that are mutant for a gene product X die. Variants of the X gene that were reintroduced through homologous recombination indicate that variant proteins that lack the C-terminal region of the protein cannot rescue the lethality (did not allow them to live), and always localized to the cytoplasm. Those that retained the C-terminal region rescued the lethality (conferred viability!) and were consistently localized within the nucleus. RNA-seq analysis of the mutant cells vs the wild-type cells indicated that the expression of many genes that are essential for neural function was reduced in the knock-out mutant cells. The N-terminal region of Protein X is 100% conserved between mouse and humans at both the amino acid and the nucleotide level. The predicted mouse mRNA sequence is shown below where the AUG corresponds to the translational start site (AUG). 5’-AUGUUUACAGAGGGGAAU... -3’     d) What motif could be present to direct this protein to its correct destination?  e)…

Please answer both

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Knock out mice that are mutant for a gene product X die. Variants of the X gene that were reintroduced through homologous recombination indicate that variant proteins that lack the C-terminal region of the protein cannot rescue the lethality (did not allow them to live), and always localized to the cytoplasm. Those that retained the C-terminal region rescued the lethality (conferred viability!) and were consistently localized within the nucleus. RNA-seq analysis of the mutant cells vs the wild-type cells indicated that the expression of many genes that are essential for neural function was reduced in the knock-out mutant cells. The N-terminal region of Protein X is 100% conserved between mouse and humans at both the amino acid and the nucleotide level. The predicted mouse mRNA sequence is shown below where the AUG corresponds to the translational start site (AUG). 5’-AUGUUUACAGAGGGGAAU... -3’ Q3a) Using a total RNA sample obtained from human cells, what is the first primer you would…