kcat is:     a measure of the catalytic efficiency of the enzyme     the rate constant for the reaction ES → E + P     the rate constant  for the reaction ES → E + S     the [S] that half saturates the enzyme     ½ Vmax

on When testing the effect of substrate concentration on the reaction rate, there comes a point at which increasing the substrate concentration has no effect on the reaction rate. That is, the reaction rate remains the same and no longer increases. Which of the statements below would explain this? Select one: O A. The enzyme is denatured OB. The substrate is denatured OC. All available enzymes are saturated or full with substrates. OD. The enzyme concentration is too high

please see attached

Based on the chart below, comment on the effect of reaction time on the reaction.