Is the DNA extracted from banana pure? Explain
Q: Based on the picture, why plasmids are used as a vector for DNA Recombination? What other vectors…
A: Ans: Recombinant DNA technology: The given process is the process of recombinant DNA technology in…
Q: How is the melting curve of duplex DNA aff ected by adding a small amount of ethanol?
A: Melting curve analysis is an assessment of the dissociation characteristics of double-stranded DNA…
Q: What is a poor transformation efficiency?
A: Transformation is a natural phenomenon mostly observe in many bacteria. It is dependent on the…
Q: ocedure to extract DNA through banana?
A: A nucleic acid called DNA is arranged into chromosomes. It has genetic data in it. Polynucleotide…
Q: Why should the extracted DNA be immersed in TE buffer
A: Introduction DNA( deoxyribonucleic acid) the natural chemical of complicated molecular structure is…
Q: Why Sanger Sequencing Dependson DNA Polymerase?
A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…
Q: what is the difference between DNA microarray and Fluorescence in situ technique? or is the…
A: Difference between FISH and Microarray Technique Fluorescence In Situ Hybridization It is possible…
Q: In making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a…
A: Introduction Enzymes play a key role in biotechnological tools which helps in various invitro…
Q: Why is it possible to visualize a PCR product on an agarose gel even if the template genome is…
A: Gel electrophoresis is a technique used in the separation of macromolecules such as proteins and DNA…
Q: What is the transformation?
A: Transformation and selection of microbes are key advances in DNA cloning. DNA cloning is the way…
Q: When making recombinant DNA, why must you use the same restriction enzyme to cut the gene of…
A: The technique of creating multiple, identical copies of a specific portion of DNA is known as DNA…
Q: Why is ligase needed to make recombinant DNA? Whatwould be the immediate consequence in the cloning…
A: DNA replication is the process of copying the DNA of the cell during cell division. DNA ligase is an…
Q: What is the purpose of using DNA polymerase from Thermus aquaticus for the polymerase chain reaction…
A: Thermus aquaticus is a heat tolerant bacterium .DNA polymerase used from Thermus aquaticus is heat…
Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?
A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…
Q: Explain the process of bacterial DNA extraction.
A: DNA extraction DNA extraction is a method of separation and isolation of pure DNA from other…
Q: What is a DNA extract?
A: DNA or deoxyribonucleic acid is a biomolecule that serves to act as genetic material for most of the…
Q: Why is Agrobacterium mediated genetic transformation described as Natural Genetic engineering in…
A: Agrobacterium is a gram-negative bacteria that is responsible for causing tumor in plants by…
Q: Why do DNA Polymerases Require a Primer?
A: DNA Polymerase DNA Polymerase is the enzyme responsible for normal DNA synthesis. Different kinds of…
Q: Explain the Cleavage of DNA by the restriction enzyme EcoRI?
A: Introduction Enzymes are the crucial biomolecules which assists in various biochemical reactions…
Q: For PCR, what property of DNA does temperature influence? Why do the “denaturation” and “annealing”…
A: PCR stands for polymerase chain reaction. It is an in-vitro laboratory technique used for generating…
Q: Why were both “+ DNA” and “- DNA” mixtures prepared and plated?
A: Both “+DNA” and “-DNA” mixtures prepared and plated because in the transformation protocol “+DNA”…
Q: If the cloning was successful and the plasmid contained the elements needed for blue-white…
A: The screening of transformants after the cloning is a critical step as it allows the identification…
Q: Can DNA be isolated from beef? Discuss the process briefly
A: The purification of DNA from the collected sample of DNA with the help of the various methods and…
Q: Explain the Powerful Technique for Copying DNA ?
A: Introduction: Multiple copies of DNA can be synthesized in the in-vitro medium with the help of the…
Q: In producing genetically engineered human insulin in bacteria, why is it important to use the…
A: Insulin is a natural peptide hormone produced by the beta cells of the pancreas in response to high…
Q: In this picture of gel electrophoresis of DNA, which sample contains the longest/largest piece of…
A: DNA (Deoxyribonucleic acid) is a double helical structure that consists of nucleotide. Nucleotide…
Q: Why is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I, in DNA…
A: A protein fragment that is obtained when the DNA polymerase I of E.coli is enzymatically cleaved…
Q: Ehat primer sets could be amplify the following DNA sequence? AATACGTCGCATGGggatccttttttatgcatg
A: Amplification of DNA sequence is done by the polymerase chain reaction. With the help of DNA…
Q: What is the purpose of the dideoxynucleotide triphosphates in the Sanger sequencing reaction?
A: In 1977, Sanger proposed chain termination method of DNA sequencing. He used ddNTP or…
Q: Is the DNA soluble in the aqueous solution or alcohol?
A: Solubility of the substance depends on molecular polarity. Polar molecules are hydrophilic…
Q: Why is the use of temperaturestable DNA polymerase an important factor in the polymerase chain…
A: The polymerase chain reaction (PCR) is the process of artificially synthesizing multiple copies of a…
Q: What does A230 indicate, in assessing extracted DNA quality?
A: Option C Extraction contaminants
Q: Explain about dideoxy chaintermination sequencing ?
A: Dideoxy chain termination method is also called as Sanger sequencing. This method can be designed…
Q: When is the transformation factor used?
A: According to Griffith's experiments, based on the bacterial transformation through the Streptococcus…
Q: Why do alcohol and water separate in DNA extraction process?
A: DNA extraction is defined as a process that is used widely for carrying out the purification fo DNA.…
Q: What is the function of each reagent used in the extraction of DNA from banana? •Saltwater…
A: The extraction of DNA from any cell involves the following steps: Homogenization of the cells to…
Q: When isolating DNA, why is it helpful to control environmental conditions?
A: The ability to extract DNA is of primary importance to study the genetic causes of disease and for…
Q: Why is there a need for replica plating? How is it performed?
A: Hi! Thanks for your question. But as you have posted multiple questions, I am answering the first…
Q: The purpose of using soap in the DNA extraction activity using frozen strawberries is to
A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…
Q: What is the purpose of adding table salt (NaCl) to the DNA extraction buffer?
A: The deoxyribonucleic acid (DNA) carries the genetic information and is usually present in the…
Q: What causes the separation of DNA fragments during agarose gel electrophoresis?
A: The technique of Gel electrophoresis is used or the separation of DNA fragments or macromolecules…
Q: Explain why DNA fragments migrate in a gel electrophoresis. Which fragments migrate farthest: large…
A: Introduction :- The technique of gel electrophoresis is used to separate DNA fragments based on…
Q: What is the transformation factor?
A: The process in which the genetic material formed of the naked DNA (deoxyribonucleic acid), which is…
Q: If insert DNA was obtained by PCR using Taq DNA polymerase, what shape does the end of this DNA…
A: Taq polymerase is basically a thermostable polymerase that is obtained from the bacteria called as…
Is the DNA extracted from banana pure? Explain
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