Explain why the absorption of UV light by double-stranded DNA increases (the hyperchromic effect) when the DNA is denatured.

Please help with 2a) 2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA PolymeraseIII, that are active during prokaryotic DNA replication. Suppose you generated a mutant E. colistrain in which DNA Polymerase III was inactivated (all its enzymatic activities were non-functional) - assuming that all the other enzymes involved in replication remained fullyfunctional, how would DNA replication in these mutant cells without DNA Pol III differ fromDNA replication in normal E. coli? Briefly explain why you would expect to see thatchange/those changes in DNA replication in the mutant cells.

Why does a fiber ( double stranded molecule or duplex) of DNA shorten upon drying?

Match the bold DNA repair response(s) to the triggering type of DNA damage. Homologous Recombination Mishmatch Repair Base Excision Repair Nucleotide Excision Repair Non-homologous End Joining Single-strand DNA breaks Removal of repair lesions such as photoproducts caused by UV including Thymine dimers Double-Strand Break repair mechanism which is an accurate repair mechanism without any introduction of insertions or deletions. It requires a sister chromatid as a template. This repair mechanism uses just DNA glycosylase to remove Uracil (no other enzymes or complexes are required) then DNA polymerase can use the template stand to add the complementary base where the Uracil has been removedInterstrand Crosslink Repair This repair mechanism is used to recognize and repair mis-incorporation of base that can arise during DNA replication. Removal and replacement of modifying bases such as Uracil, 8-hyroxyguanine and others. Double-strand Break that is termed as “Quick and Dirty” as it is…