What is a poor transformation efficiency?

Why do longer DNA molecules move more slowlythan shorter ones during electrophoresis?

Do restriction enzymes always cut the DNA at the recognition sequence?

Which of the following plates could represent the results of the transformation experiment with PGLO when plated on LB/Amp, if the experiment was successful? Refer to the letters on top of the image. Ignore the letters at the bottom. A B OB O none of the choices are correct

what is the purpose of calcium chloride in the transformation process?

Taxol is a compound used in cancer treatment. You are working for Genentech on a project to optimize the production of taxol purified from recombinant E. coli bacteria.  You have two new strains of SuperGro E. coli:  Strain A and Strain B, that you have engineered to express taxol. You want to know which of the two SuperGro E. coli strains is better to use for purifying taxol based on the amount you purify (measured by final concentration of protein in mg/mL). You also want to know which growth media (LB Media or SOC Media) results in a higher amount of purified taxol. You collect data and plot the average final concentration of taxol from each experimental condition in the graph below. Use the approach we discussed in class and write your analysis and interpretation of the data (describe the graph, describe the data, and interpret the data). Make sure to give clear and complete descriptions.  A. Describe the graph: B. Describe the data: C. Describe the interpretation:

PCR is a molecular biology technique where template DNA is amplified using a primer and oligonucleotides. The reaction is catalyzed by a thermostable DNA polymerase and in a particular reaction, the template strands are denatured at 95˚C. For strand hybridization, the melting temperature is 55˚C. What do you predict about the average duration of H bonds at the high temperature in comparison to the low temperature?