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- b) Draw the single-stranded segment correctly.Cells that continue toexpress telomerase include which cells?2c) If the whole potoroo genome is 4.2 x 10' bp, and the highlyrepetitive DNA in the potoroo genome is composed entirely ofcopies of the sequence 5'AAGACT' and its complement, howmany copies of this sequence are present in the potoroogenome?
- Provide a brief summary of the Sanger sequencing method.How do these designed nucleases modify the genomic DNA sequence?12) Draw a yeast knockout cassette. Label all required sequence features. a) Draw the target chromosome if recombination only occurs at one region of homology. b) Draw the target chromosome if recombination occurs using both regions of homology.
- a. Why doesn't Dpnl digest the mutagenized DNA? b.What is the approximate size, in base pairs, of the pQE.1-CRYGD plasmid?a. Why do bacteria make restriction endonucleases? b. What is it about the endonucleases that prevents bacteria from destroying their own DNA?Explain how DNA polymerase and topoisomerase 2 contribute to replication in E.coli and what is the role of the role of the metal ions in the polymerase activity. B)How does the use of an RNA primer rather than a DNA primer affect the fidelity of DNA replication in E.coli?
- 1c) During DNA replication, both positive and negative supercoiling is introduced in the DNA being replicated. Name the enzymes that introduce supercoiling into DNA during replication; please clearly indicate which enzyme(s) introduce positive supercoiling and which enzyme(s) introduce negative supercoiling.State the difference between prokaryotic and eukaryotic DNAreplication.describe Deletion-insertion polymorphisms (DIPs)