In replication, if reading the template leading strand from left to right reads in the 3' to 5' direction, which of the following would also read left to right in the 3' to 5' direction (hint: draw it out)? The template lagging strand The Okazaki fragments None of the other choices

Q: Which activity of E. coli DNA polymerase I is responsible for proofreading the newly synthesized…

A: The first known polymerase is the DNA Polymerase I. It is the enzyme that participates in…

Q: Which of the following enzymes adds incoming deoxyribonucleotides/deoxyribonucleoside triphosphates…

A: DNA replication is defined as the process by which a molecule of DNA is replicated or duplicated.…

Q: Briefly describe the importance of origin of replication in a cosmid and where are the cos sites…

A: Cosmid vectors are hybrids between plasmid and phage λ vectors.  Cosmid vectors are used in…

Q: What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that…

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…

Q: During a PCR reaction, the step in the cycle where dNTPs are added to the primer 3’ ends to fill in…

A: Kary Mullis created the groundbreaking technique known as PCR (Polymerase Chain Reaction) in the…

Q: Which of the following enzymes at the bacterial replication fork hydrolyze ATP for their activity?…

A: Introduction :- DNA primase, a type of RNA polymerase, is an enzyme that aids in DNA replication. A…

Q: Using the picture below, match each letter (A-E) to 5' or 3' DNA polymerase molecule Parental DNA…

A: As the options are not visible, we are answering the question based on the general principle of DNA…

Q: True or False Replication of genetic material means that the genetic material is recreated to make…

A: The transfer of information from DNA to proteins is a familiarly known fundamental process that…

Q: Statement Replication Transcription The new strand is made 5' to 3'. The new strand is made 3' to…

A:

Q: What is the difference between DNA proofreading and DNA repair? In 3-4 sentence

A: Deoxyribonucleic acid (DNA) is a biomolecule found in nearly all living organisms. DNA used as…

Q: In the gel electrophoresis: the mutant would just run the same as the open circle DNA if it simply…

A: In gel electrophoresis, the behavior of mutant DNA in comparison to open circle DNA and the presence…

Q: Specifically, why is the primer needed in order for DNA ploymerase to start synthesis? A ) primers…

A: Always replication, Transcription starts from 5' to 3'. Addition of nucleotide takes place at 3'-OH.

Q: The DNA sequence below is used by the primase to synthesize a primer. What is the sequence of the…

A: The primer of this sequence will be complementary to it. The complementary bases are as follows: A…

Q: In the diagram shown below representing a replisome, the portion represented by the letter functions…

A: Ans. The method of DNA replication is a process in which double-strand DNA molecule copying in order…

Q: there is nucleotide selectivity during replication, why do polymerase choose a wrong nucletide and…

A: A nucleotide is the basic building block of nucleic acids, such as DNA and RNA. It is composed of…

Q: the RNA primer at the beginning of each Okazaki fragment is removed by : A-DNA polymerase I  B-DNA…

A: Replication Replication is defined as the process of synthesis of new DNA from the existing DNA…

Q: During a typical gel electrophoresis set up (negative pole at the top), which DNA fragment would be…

A: Gel electrophoresis is the technique for the separation of DNA fragments on the basis of size and…

Q: Is the template strand read in the 5′ to 3′ or the 3′ to 5′ direction?

A: DNA (deoxyribonucleic acid) replication is a semiconservative process where each strand in the…

Q: Which of the following processes of genetic information flow can occur under lab conditions, but has…

A: The DNA (deoxyribonucleic acid) is the double-stranded molecule which is the genetic material in…

Q: In the Meselson Stahl experiment differentiating the possible modes of replication, how many rounds…

A: They demonstrated that DNA replicated semi conservatively,  meaning each strand in a DNA molecule…

Q: Which of the following has 5’ to 3’ exonuclease activity? DNA polymerase I DNA polymerase III DNA…

A: The 5'-3' exonuclease activity's primary objective is to eliminate the RNA primers from newly…

Q: What is the following molecules not required for DNA synthesis during S- phase? * O RNA primer…

A: DNA replication is the process by which new DNA is synthesized from the old DNA. In case of…

Q: Why and how do the polymerase go backward from state 2 (post-translocation state) to state…

A: During replication and transcription, the polymerase enzyme moves along the DNA template to…

Q: Match the following (most appropriate combinations): helicase Topoisomerase DNA Polymerase III DNA…

A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains which wrap around…

Q: Clearly diagram rolling circle replication. Add 5’ and 3’ and show the direction of replication with…

A: A type of unidirectional nucleic acid replication involves the rolling circle replication where the…

Q: Illustrates a Model of Replication Fork Instructions for Making a Model of the Replication Fork 1.…

A: DNA is vital for all living beings – even plants. It is important for inheritance, coding for…

Q: Figure 2 illustrates the important elements of a cosmid. a) Briefly describe the importance of…

A: In the recombinant DNA technology the foreign DNA or target DNA is inserted into the vector then the…

Q: If the DNA that is about to be replicated has the following sequence: 5' ATTGCACTTAGGAATT 3' 3'…

A: INTRODUCTION The genetic material contained in all living things is called DNA.It is a…

Q: Recall that Meselson and Stahl used heavy nitrogen (N15) and light nitorgen (N14) to determine the…

A: Introduction: DNA, or DNA, could be a kind of genetic material that transmits information from one…

Q: Which of the following processes of genetic information flow can occur under laboratory conditions,…

A: The DNA (deoxyribonucleic acid) is the double-stranded molecule which is the genetic material in…

Q: strand) tom strand) er a few rounds of replication, what will the sequence of the mutated top strand…

A: In the given DNA sequence, the original top strand was5'- TAGCATGCAA - 3'.When guanines in this…

Q: Which of the following enzymes ensures that the correct base of a deoxynucleotide for growing the…

A: DNA replication is the process of duplicating a DNA molecule. Every time a cell divides, it must…

Q: lication fork. O Top; Top ) Which template strand (top or bottom) is the lead strand? D) Which…

A: No DNA synthesizing enzyme that is no DNA polymerase can initiate the synthesis of the new DNA. It…

Q: An experiment is performed with Primase, using a specific DNA template. In this experiment, you…

A: Radiolabeled nucleotides are used for the detection of specific nucleic acid sequences. For…

Q: As the strands are synthesized in replication, which of the following is true?   the leading strand…

A: Replication:- process of formation of replica's of DNA in a semiconservative manner.  Bidirectional…

Q: What is the difference between semi-conservative replication and dispersive replication?

A: DNA  The DNA or deoxyribonucleic acid is made up of four nucleotide sequence adenine, guanine,…

Q: In E. coli, the leading strand is primarily synthesized by:       DNA polymerase ε   DNA polymerase…

A: DNA replication is a process in which the DNA makes multiple copies of itself.  The cell must…

Q: Why is a clamp loader necessary in replication?

A: Clamp loader was identified as DNA polymerase processivity factors. Clamps not only increase the…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.