Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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The semi-conservative model |
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- K The template (antisense) strands of two complete (double-stranded) DNA molecules have the base sequences shown in the table below. Two replication experiments are done with each molecule: 1. In Experiment #1, samples of each DNA molecule are incubated with radioactive cytosine, along with appropriate replication enzymes, ATP, adenine, thymine, and cytosine. Experiment #1 is stopped when each DNA molecule has replicated once. 2. In Experiment #2, all the DNA molecules from #1 are purified, and then incubated with with a similar reaction mixture - except non-radioactive cytosine is used this time. Experiment #2 is stopped when each DNA molecule has replicated one more time. Predict the percentage of DNA in each sample that is radioactive after each experiment. Round your answers to the nearest percent. DNA template strand sequence 3'-GAAGCC -5' 3'-ACCTTA-5' Explanation Check - % of DNA radioactive after... Experiment #2 Experiment #1 0% % % % Q Search X S © 2023 McGraw Hill LLC. All…arrow_forwardMatch the definition on the left with the term on the right Tightly hypercolled DNA that is not in use Loosely colled DNA that is currently being used a) Chromatin b) Chromosomearrow_forwardIn 1958, Meselson and Stahl conducted an experiment to determine which of the three proposed methods of DNA replication was correct. Identify the three proposed models for DNA replication. Conservative xxx xxx XXxxx xx xa xxxx xxx xxx Semiconservative Answer Bank xxx XXXX < Dispersive xxxxxxx XxXxXxXxxarrow_forward
- You transform 500 uL of cells with 2.5 ug of plasmid DNA, and then you take 100 uL and spread that on a plate. 3 days later you count 11 cfu. What was the transformation efficiency? use the following formula: TE =(VP/VTT) * (cfu/ug of DNA)arrow_forwardAn Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that the DNA remains double- stranded at the replication forks. What is the likely target affected by the temperature-sensitive mutation? DNA Ligase DNA polymerase lII primase DNA gyrase Single-stranded DNA-binding protein (SSB)arrow_forwardDoes E. coli chromosomal replication always start at one particular site? What is called? If you were given the DNA sequence of E. coli chromosome, would you be able to identify where E. coli chromosomal replication starts? What is the end of E. coli chromosome replication?arrow_forward
- Draw a diagram of a single replication fork. Label the following on your diagram: 1. All 3’-OH ends 2. All 5’-phosphate ends 3. leading strand (s) 4. lagging strand (s) 5. Okazaki fragments (draw 3) ❏ RNA primer(s) 6. direction of replication ❏ replisome(s) 7. origin (s) 8. terminus (i)arrow_forwardIn the experiment of Mesel- Question 3 son and Stahl on DNA replication, if the cells are cultured for many generations in a medium containing heavy nitrogen (N 15) and thereafter transferred to a medium containing only light nitrogen (N 14), what percentage of double- stranded DNA is composed of two heavy chains after one generation? A 25 B 75 C 0 D 50 E 100arrow_forwardSuppose a researcher conducts matings between three Hfr bacterial strains and an F− strain. The table shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Identify the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin.arrow_forward
- If you had a mixture of single-stranded DNA fragments, all 4 deoxyribonucleoside triphosphates, and DNA polymerase III. Would the single-stranded DNA fragments be able to be replicated? No, DNA polymerase I is needed No, ribonucleoside triphosphates are needed Yes, DNA polymerase III would be able to copy the DNA fragments from the 3' to 5' end by attaching the complementary deoxyribonucleosides No, DNA polymerase III would not be able to bind to the DNA fragment to start replicationarrow_forwardYou have two tubes, each with a pair of DNA fragments inside them. Tube #1 has fragments that are 500bp and 1000 bp in length. Tube #2 has fragments that are 7500bp and 8000bp in size. If you were to perform agarose gel electrophoresis and run the contents of each tube in two separate lanes on the same gel, what would you expect to see? O That the difference between the distances migrated by the two fragments in Tube #1 was much greater than the difference between the distances migrated by the two fragments in Tube #2. O That the difference between the distances migrated by the two fragments in Tube #1 was the same as difference between the distances migrated by the two fragments in Tube #2. O That the difference between the distances migrated by the two fragments in Tube #1 was much less than the difference between the distances migrated by the two fragments in Tube #2. O It is not possible to estimate what we would expect to see.arrow_forwardOn paper, replicate the following segment of DNA: 5’ A T C G G C T A C G T T C A C 3’ 3’ T A G C C G A T G C A A G T G 5’a. Show the direction of replication of the new strands and explainwhat causes lagging and leading strands to show different patternsof replication.b. Explain how this is semiconservative replication. Are the newstrands identical to the original segment of DNA?arrow_forward
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