The DNA sequence below is used by the primase to synthesize a primer. What is the sequence of the primer? 5’-ACGTGCT-3’

Q: Which RE’s from table below have a 5’ overhang? Which ones have a 3’ Overhang?   AccI GT^CGAC…

A: Restriction enzymes are the specific enzymes that recognize and cut at specific sites on nucleotide…

Q: The following DNAs were incubated with E. coli DNA Polymerase I and dNTPs. Write the products…

A: DNA replication is the process by which new DNA is synthesized from the old DNA by semiconservative…

Q: The 5'-3' exonuclease activity involves all the following EXCEPT: o Both ribonucleotides and…

A: DNA replication is a complex process that occurs during the cell cycle and allows a single cell to…

Q: please list all possible products in a sequencing reaction using ddGTPA as a terminator based on the…

A: Ans: Chain termination sequencing: It is sanger sequencing method developed by Frederick sanger. The…

Q: Why do adult human cells (other than germ cells and stem cells) NOT express the enzyme telomerase?…

A: Telomerase expression is required for cell immortalization ( in cancer cells) and long term tumour…

Q: DNA polymerase I DNA polymerase II DNA ligase Primase RNA primer 5' Lagging strand 3' 3' Okazaki…

A: The process of copying of double stranded DNA by the application of several Enzymes and proteins…

Q: In a random sequence consisting of equal proportions of all four nucleotides, what is the average…

A: Restriction enzymes are natural occurring enzymes which are mainly found in prokaryotic organisms. A…

Q: What is the importance of the 3' to5' exonucleasevactivity of DNA polymerase

A: Enzymes serve as biological catalysts that fasten the rate of reaction by lowering the activation…

Q: What is meant by a primer, and why are primers necessary for DNA replication?

A: Primers are short DNA or RNA sequences complementary to the existing DNA strands. Without a primer a…

Q: A virus with a circular double-stranded DNA chromosome contains approximately 10,000 bp. You want to…

A: To construct a map of the viral chromosome based on the cleavage sites of the restriction…

Q: In a typical microbiology laboratory, reasons for no bands from a gel of a polymerase chain reaction…

A: Polymerase chain reaction (PCR) is the in-vitro amplification of the DNA sequences. The process…

Q: What distinguishes topoisomerase type I from topoisomerase type II (“gyrase”) in E. coli bacteria?

A: Those in science which participate in overwinding aur underwinding of DNA are called topoisomerases.…

Q: Explain TWO (2) differences between two commonly used ligases; F. coli DNA ligase and T4 DNA ligase.

A: During DNA cloning process , various restriction enzymes like exonucleases and endonuclease as well…

Q: You plan to clone exon 11 of the HEXA gene (Section C) into the multiple cloning site (MCS) of the…

A: Answer well explained above

Q: This is a restriction map for the 250 base pair plasmid pSage. Restriction sites for the restriction…

A: Restrictions endonucleases are enzymes that cut down the DNA into fragments near or at the site of…

Q: When circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed…

A: It is related to molecular biology in which we use restriction enzymes an important tool for

Q: Although DNA polymerases require both a template and a primer, the following single-stranded…

A: DNA polymerases require both a template and a primer to synthesize DNA. However, some…

Q: E. coli DNA polymerase V has the ability to bypass thymine dimers. However, Pol V tends to…

A: DNA polymerase is an enzyme, which helps to synthesize new complementary strands with the…

Q: The restriction endonuclease NotI recognizes the octanucleotide sequence GCGGCCGC. Calculate the…

A: Restriction endonucleases are enzymes that cut DNA sequences at a specific site known as a…

Q: How often, on average, will the endonuclease AluI, which cleaves the sequence 5’ AGCT 3’, cut normal…

A: Enzyme restriction, also referred to as endonuclease restriction, is a protein formed by bacteria…

Q: A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease.…

A: Because plasmid is a circular DNA molecule, this is the case. It becomes linear but not fragmented…

Q: DNA

A: Answer--

Q: The sequence of the transcript is 5'-GGUUACAUUC-3'. The sequence of the DNA template would be:…

A: Transcription is a biological process that occurs in the cells of living organisms, where genetic…

Q: 1) The DNA fragment shown in Figure 1 is cleaved by the restriction enzyme EcoRI as indicated. The…

A: Q 1.) :- Answer.Based on the given information, the DNA fragment shown in Figure 1 is cleaved by the…

Q: What is meant by a primer, and why are primers necessary for DNA replication?

A: DNA replication produces new DNA molecules using the existing DNA strand as a template.

Q: In producing genetically engineered human insulin in bacteria, why is it important to use the…

A: Insulin is a natural peptide hormone produced by the beta cells of the pancreas in response to high…

Q: Budding yeasts such as S. cerevisiae exhibit telomerase activity throughout their life cycles,…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Which of the follwing enzymes adds incoming deoxyribonucleotid triphosphates to the 3' OH of the…

A: Deoxyribonucleotides or deoxyribonucleoside triphosphates (dNTPs) are the building blocks of DNA.…

Q: For the following sequence, what is the Tm? 5'-AGCTACGATCAGGTCA-3'

A: Polymerase chain reaction (PCR) is a technique to amplify the sequence of DNA. A large quantity of…

Q: Which of the following represents a base analog acting as a mutagen? O ethidium bromide O guanine O…

A: In hereditary qualities, a mutagen is a physical or compound specialist that changes the hereditary…

Q: Describe precisely the catalytic activity of DNA polymerase I that removes RNA primers. O 3' to 5'…

A: An enzyme called DNA polymerase I (also known as Pol I) takes involved in bacterial DNA replication.…

Q: In the catalytic center of DNA polymerases, the presence of Mg2+ ions and aspartate residues close…

A: DNA replication is the process by which DNA is duplicated. Duplicated DNA is transferred to the…

Q: he sequences below indicated the 6bp recognition site for the restriction enzyme EcoRI. The lines…

A: EcoRI is a restriction endonuclease that is isolated from E. coli and is used in the gene cloning…

Q: The restriction enzymes KpnI and Acc65I recognize and cleave the same 6-bp sequence. However, the…

A: To answer this question we should have knowledge of Biotechnology

Q: Given the DNA sequence of the restriction enzyme: gi|6329444|dbj|AB034757.1| Hynobius retardatus…

A: Restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence…

Q: termini). What type of mutation is each? 6.a. Mutated DNA Template Strand #1: 3'-TACTGTC TGACGAT…

A: DNA It is a double helix structure that constitutes two polynucleotide chains wound around each…

Q: Ehat  primer sets could be amplify the following DNA sequence?   AATACGTCGCATGGggatccttttttatgcatg

A: Amplification of DNA sequence is done by the polymerase chain reaction. With the help of DNA…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.