In order to manufacture insulin for patients with diabetes, scientists create recombinant DNA by inserting a human insulin gene into bacterial DNA, and have the bacteria produce human insulin. Using knowledge about gene expression, what must the scientists do to the DNA sequence of the human insulin gene BEFORE inserting into the bacteria, in order to have them produce the same insulin sequence that humans would produce? Explain.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
In order to manufacture insulin for patients with diabetes, scientists create recombinant DNA by inserting a human insulin gene into bacterial DNA, and have the bacteria produce human insulin. Using knowledge about gene expression, what must the scientists do to the DNA sequence of the human insulin gene BEFORE inserting into the bacteria, in order to have them produce the same insulin sequence that humans would produce? Explain.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
