)A scientist compares the sequence of a disease gene and a healthy gene and finds that these two genes are identical except for one G in place of a T at position 256 in the gene.  This type of mutation is a(n): Select one: a. deletion b. insertion c. silent mutation d. frameshift e. substitution mutation

Define all the terms we use to describe the different types of mutations. For example, true-back mutation, point mutation, deletion, insertion, frame-shifting, conditional, silent,

Which of the following types of mutations would be predicted to harm an organism? explain your answers. A. insertion of a single nucleotide near the end of the coding sequence.B. removal of a single nucleotide near the beginning of the coding sequence. C. Deletion of three consecutive nucleotides in the middle of the coding sequence. D. Deletion of four consecutive nucleotides in the middle of the coding sequence. E. substitution of one nucleotide for another in the middle of the coding sequence.

we will analyze the Breseq data. If you see the symbol /+', it means that the mutation is located

To quantify the transcription product of a specific gene in E. coli, you may use (select all correct answers) O a. Northern blotting O b. Topoisomerase I O C. Primer extension O d. Foot-printing

The amino acid sequence of part of a protein has beendetermined:N . . . Gly Ala Pro Arg Lys . . . CA mutation has been induced in the gene encodingthis protein using the mutagen proflavin. The resultingutant protein can be purified and its amino acidsequence determined. The amino acid sequence of themutant protein is exactly the same as the amino acidsequence of the wild-type protein from the N terminus of the protein to the glycine in the preceding sequence. Starting with this glycine, the sequence ofamino acids is changed to the following:N . . . Gly His Gln Gly Lys . . . CUsing the amino acid sequences, one can determinethe sequence of 14 nucleotides from the wild-typegene encoding this protein. What is this sequence?

Which of the following is NOT common to all repair mechanisms? A. Detection of the lesion B. Removal of the damaged DNA sequence/ nucleotide/ base C. Removal of free radicals at the site of injury D. Repair of the lesion E. Involvement of enzymes in removing the lesion and/or repair of the damaged sequence/nucleotide/base.

What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases?   a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d.   The DNA band detected by Southern blot is different from that by Northern blot

Albinism is a condition where individuals can't make melanin pigment. The affected gene encodes for tyrosine aminotransferase, a key enzyme in melanin production. If you analyze the DNA of an albino individual, of the following mutations, which one is the least likely mutation responsible for the albino phenotype?   A. A substitution of A to G at 3' splicing site.   B. A deletion in the TATA box region.   C. An insertion after the start codon.   D. An extra stretch of TTAATT in intron 1.

Which of the following types of mutations will have the most devastating effect on a cell?A.frameshift mutationB. missense mutationC. nonsense mutationD. silent mutation