If you had a cell suspension of 2.5 x 10^4 cells/mL and plated 100uL into each well (4 wells), how many cells were added per well?

Q: A radiograph was produced using 80 kVp, 800 mA, .75 seconds and a 72 inches SID. In order to…

A: Given information The radiograph was produced with values: Kilovoltage- 80kVp Quantity of x-rays -…

Q: Why does counting 5 Boxes in each chamber of the hemacytometer gives you the number of cells in a ul…

A: Hemacytometer is a medical instrument used to measure the concentration of blood components in a…

Q: How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.

A: In scientific research and experimentation, it is often necessary to dilute substances to achieve…

Q: Bradford protein assay data: Absorbance Readings at 595nm You will need to graph this 0.104 for P1…

A: Bradford assay is extensively applied for estimating unknown protein sample concentration.…

Q: 10ul of the original sample was transferred to 90ul diluent. Then, the mixture was mixed with 100ul…

A: Hemocytometer is blood cell counting device. It is usally cposed of thick glass on which square…

Q: If growth had been present on the –pGLO side of the LB/amp plate, what might have been a reason for…

A: Antibiotics are antimicrobial substance that acts against bacteria. Antibiotic resistance is the…

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: Occurred in her cells? (Unfortunately her parents have passed away.) I think these genes are…

A: CRISPR stands for Clustered regularly interspaced short palindromic repeats. It is a system of…

Q: Using a diagrammatical presentation show how serial dilution was performed if the final volume in…

A: Serial dilution is very useful process for diluting the sample and it is also helps in the…

Q: You are

A: Introduction: For incorporating more solvents, dilution is the process of lowering a solute's…

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: How would you determine the contents of the test tubes if all of the samples were transparent?

A: The study of chemical reactions that occurs within living organisms is called biochemistry. This…

Q: You start working in a new laboratory. When you arrive, you find a few vials on the bench. The…

A: A 1 molar(1M) solution contains the molecular weight of a substance(in grams) in 1L of solution. The…

Q: A physician orders an IV of 40mEq potassium chloride in 1,000 ml NS to run at 125mL/ hr. How many…

A: There are different routes for the administration of medicines. The oral route, subcutaneous,…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: but why is the answers all x 1000 at the end? for example day 1 is 2.025×1000=2025CFU/ml. should…

A: Answer.) :- When working with colony-forming units (CFU) in micro biology, we often need to dilute…

Q: Refer tó the image below to answer this question. You have a bacterial culture with a concentration…

A: Serial dilution is the dilution of a substance in the given solution in a stepwise manner. Serial…

Q: The Leica microscope that we will be using for histology lab has four different objective lenses and…

A: Microscopes are used to magnify an object. In biology laboratories compound light microscopes are…

Q: How did you get the dilution factor of 10^4? I did initial volume of 1mL/ final volume of  9+1…

A: Dilution factor is the reciprocal of dilution. This means that dilution factor is Vf/ Vi.

Q: You are counting white blood cells in a freshly drawn blood sample. You dilute the blood 1/20 in 5%…

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Q: You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and…

A: A colony-forming unit (cfu) is a unit used in microbiology to estimate the number of viable bacteria…

Q: When doing a routine differential count for a CBC how many cells are counted?   a) 50 b 75 c 100…

A: CBC It is complete blood count which refers to the set of tests that gives the details of cells in…

Q: With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable…

A: Bacteria are the prokaryotic, unicellular organisms that are sometimes pathogenic in nature. The…

Q: An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100…

A: Serial dilution is used to find the concentration of bacteria in the sample in case of greater…

Q: Using the fraction conversion method solve the following Convert 500 mL=______qt

A: Millilitres to quarts:  Both quarts and millilitres are units  used to measure volume. 1 millilitres…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: What was the initial pH of the milk? 07 4 11 2

A: As the milk ages , fermentation done by lactic acid bacteria accumulates lactic acid which makes the…

Q: If you performed the serial dilution using 0.9ml of tapwater originally and mixed and transferred…

A: A serial dilution is one of the techniques which is useful in microbiology, serology, and other…

Q: If an OD=1.0 culture yielded a plate count of 2.2 x 107 cfu/mL. What is the cell count of an OD=0.5…

A: Answer: The relationship between colony-forming units per millilitre (cfu/mL) and optical density…

Q: Upon dislodging cells from the plate, you had the following cell counts using 4 different squares of…

A: Hemocytometer: It is a device first invented by " Louis charles malassesz". It is used to count the…

Q: You want to pipette 250uL of a sample in the lab. Which pipetter do you use? O p20 O p200 Op2 O…

A: Pipetter It is a laboratory tool commonly used in chemistry, biology and medicine to transport a…

Q: Chromatography is not used to separate mixtures. O True O False

A: Introduction There are various biochemical  techniques which is used to separate the components of…

Q: You are using a spectrophotometer to see whether you accurately performed a serial dilution of a…

A:

Q: To make 500ml of 1xBuffer, measure __ML of 5x stock and add _­__mL water to make the total volume…

A:

Q: 1/2 dilution

A: Dilution solution contains stock solution or solute and a diluent also known as solvent. Since we…

Q: What occurred when the blood was mixed with each solution, distilled water, ISO 0.9% and ISO 12.5%…

A: Osmosis is a latent process and results without any expense of power. It includes the transfer of…

Q: The physician ordered 700ml of a special electrolyte solution to flow at 75 gtt/min. The drop factor…

A: The nurse should follow the "5 Rights" while administering the medication. They are Right patient,…

Q: A bacterial culture has a concentration of 3.2 x 108 cells /mL.  You dilute this culture as follows:…

A: CFUs are a measure of the number of progenitors present in a given population of cells; if an…

Q: olds 950 milliliters (m

A: Milliliter literally means one thousand of a liter, it written as ml.  One milliliter 20 drops and…

Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

A: Introduction: A CFU stands for colony-forming units. It is a unit that we use for estimating the…

Q: You are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer…

A: Dilution is a process by which solution (water or diluents) are added to the solvent (main solution)…