An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100 dilutions. Exactly 1 mL from the last dilution was used to prepare a pour plate. After incubation, how many colonies were present on this plate?

Q: A food sample with an unknown number of contaminating bacteria was serially diluted to 10-6. A…

A: Serial dilution of a food sample containing unknown quantity of contaminating bacteria was performed…

Q: What is the purpose of the RPR (RAPID PLASMA REAGIN )

A: Since you have asked multiple questions, we will solve the first question for you, If you want any…

Q: how does the diameter of the column affect the flow rate of the elution in gel filtration…

A: Introduction Gel Filtration Chromatography IsA Type Of Size Exclusion Chromatography, Which Can Be…

Q: How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.

A: In scientific research and experimentation, it is often necessary to dilute substances to achieve…

Q: If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original…

A: CFU stands for colony-forming unit that gives rise to a particular colony. It is generally assumed…

Q: One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies…

A: A colony-forming unit, or CFU, is a measurement of the concentration of microorganisms in a test…

Q: to dilute a sample by 1/3. For a final volume of 27 ml, how much sample would you add to how much…

A: The concentration of the solution fluctuates as it is diluted, but the number of moles of reactant…

Q: My question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium…

A: Casein is the major protein component found in milk. It is generally a phosphoprotein which is…

Q: 1. A plate with a final dilution factor of 107 produced 210 colonies. a) What was the original…

A: Dilution is the process of diluting or mixing two or more substances or compounds; it is also known…

Q: a food sample with an unknown number of contaminating bacteria was serial diluted to 10-6. the 10-6…

A: Serial dilution is a process of diluting a particular sample in a solution. The purpose of doing a…

Q: 10ul of the original sample was transferred to 90ul diluent. Then, the mixture was mixed with 100ul…

A: Hemocytometer is blood cell counting device. It is usally cposed of thick glass on which square…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial…

A: Culture broth is a liquid medium that is used to grow microorganisms, such as bacteria, fungi, and…

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.

A: Introduction Coliform is the gram-negative, non-spore-forming and motile or non-motile bacterial…

Q: transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution…

A: b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml

Q: Calculate the final dilution in the following dilution series:  a. 1 mL in  9 mL dilution & 100uL…

A: Dilution is calculated as amount of sample transfered into the diluent divided by the total amount…

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: How would you determine the contents of the test tubes if all of the samples were transparent?

A: The study of chemical reactions that occurs within living organisms is called biochemistry. This…

Q: What are the mechanisms of samples separation work in Thin layer Chromatography?

A: Chromatography is a physicochemical technique for the division of compound mixtures, in view of the…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland…

A: The Kirby Bauer test, also known as the disc diffusion test, is a common laboratory technique for…

Q: What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate

A: The Agar plate is made from mixing agarose and a growth medium onto a petri dish. Agarose is in a…

Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…

A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…

Q: The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial…

A: Colony forming unit refers to the measurement of the number of viable cells present in per ml of the…

Q: Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media.…

A: Sheep Blood Agar Base was formed to be compatible with sheep blood and to improve organism…

Q: A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL…

A: It is given that a culture of Staphylococcus is diluted as follows:1. The original 20mL of culture…

Q: You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL.…

A: We are given a bacterial culture containing approximately 5.0 x 108 cells/mL. To identify the exact…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and…

A: A colony-forming unit (cfu) is a unit used in microbiology to estimate the number of viable bacteria…

Q: if the biuret reagent was allowed to stand for 1 hour, what would the effect on the accuracy of the…

A: * biuret test is also called as Piotrowski test used to detect presence of peptide bonds. *In…

Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

A: The following are needed for calculating the dilution factor  1) Original volume of  solution we…

Q: You have 0.05 ml of kombucha that contains microbes at a concentration of 8.2 x 10^6 CFU/ml. You…

A: Creating proper dilutions and working out the colony-forming units or CFU/ ml is one integral part…

Q: If you performed the serial dilution using 0.9ml of tapwater originally and mixed and transferred…

A: A serial dilution is one of the techniques which is useful in microbiology, serology, and other…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: ) Gel filtration chromatography on Sephadex G50 b.SDS-PAGE

A: Gel filtration: with this method we could separate  protein with respect to molecular weight in the…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: 1 ml of urine is added to 2 ml of water. 1 ml is plated. 81 colonies form. What is the CFU/ml of the…

A: CFU/ml is the indirect measurement of the microbes present in the sample. It can be related to…

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: You are using a spectrophotometer to see whether you accurately performed a serial dilution of a…

A:

Q: chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [ Choose ] chemical…

A: Bacteria are microscopic, unicellular, prokaryotic organisms found in diverse environments. They can…

Q: A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine…

A: To keep the number of colonies on the plate within a certain range, serial dilutions of culture are…

Q: Mary Jo prepared a series of diluted samples by taking 1 mL of sample and adding it to 4 mLs of…

A: Here, Mary Jo is undertaking serial dilution (i.e. a series of consecutive dilutions) comprising of…

Q: ) If you counted 500 CFU’s in the petri dish for number 6 and 5000 CFU’s in petri dish for number 7,…

A: See below section.Explanation:Detailed explanation:a): Let's break down the situation and explore…

Clinical Pharmacology

Pharmacology is a discipline of medicine that studies how medications interact with the systems and processes of living organisms, focusing on drug action mechanisms as well as therapeutic and other purposes.