Why does counting 5 Boxes in each chamber of the hemacytometer gives you the number of cells in a ul of your sample?

Q: Dr. Riggs took 1ml of whole mouse blood and added it to 10ml of PBS. You next take 0.5 mL of Dr.…

A: The first step is to calculate the average number of cells counted in the hemacytometer. The counts…

Q: Describe how to prepare and stain thick and thin blood films in a laboratory

A: Blood smears are used to identify blood cells and/or demonstrate the presence of parasites in a…

Q: The before and after fluids were observed to detect the movement of salt across the dialyzing…

A: Dialysis is defined as a type of laboratory technique where it removes the salt or reduce the…

Q: If test strips have not been stored at room temperature or are wet, they should not be used. True…

A: It is important to follow the instructions provided with the specific test strip to determine…

Q: You are the RT of the patient, you are asked to suction a newly intubated patient with a 7mm ET.…

A: Suctioning is a very common procedure done for the intubated patients. Suctioning should be done…

Q: You are looking at a specimen on low power and you cannot see details that your instructor asked you…

A: If I am looking at a specimen at low power and can't see details that my instructor asked me to…

Q: Using a diagrammatical presentation show how serial dilution was performed if the final volume in…

A: Serial dilution is very useful process for diluting the sample and it is also helps in the…

Q: Which of the following is True?a. The human stomach maintains a very high pH.b. Gastric glands in…

A: Stomach is a sac like organ. It is located between the esophagus and the small intestine. It stores,…

Q: If you didn’t get countable colonies and are not able to calculate CFU, consider the possible…

A: In laboratories,  the bacterial cultured on the agar plates are counted by the help of CFU(colony…

Q: A capillary tube of whole blood is collected and centrifuged to obtain a plasma sample. A 1 in 4…

A: Capillary tube is a cylindrical tube with a fixed diameter which may hold any type of gas, liquid,…

Q: The dot plot above shows cells that have been characterised by forward and side scatter. Forward…

A: Fluorescent dyes can be used to detect and monitor pollutants in the environment. Fluorescent dyes,…

Q: Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability…

A: The hemocytometer was first discovered by Louis Charles Malassez to count blood cells, hence, the…

Q: How is the proper amount of water to be filtered determined? Why is this determination critical to…

A: The quality of water shows the water condition including biological, chemical, and physical…

Q: Gloves are sterile if they have never been used, especially out of a newly opened box. True or False

A: Hand gloves are generally used to protect hands from heat, cold, abrasion, chemicals, and other…

Q: Explain the working principle of a flow cytometer. List its applications.

A: Flow cytometer is an analytical technique which is used for analysis of single particle or cell.…

Q: Steven is a lab research assistant. He mixed 25 µL of cells with 50 µL of cell culture media in…

A: A Hemocytometer slide is a specially designed glass slide with Neubar's rulings in the central…

Q: For the four tubes used in today's experiment (lab handout, page 23), which tube is used as negative…

A: A controlled experiment is a type of scientific test that is used by a scientist to test a…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: Why is it important to eject a small amount of blood from the syringe sample onto a gauze pad before…

A: Blood gas analysis is a diagnostic test. It gives useful information regarding the respiratory and…

Q: Despite widespread automation, why is it important for medical technologists to know the basics on…

A: Hemocytometer is a chambered device which is used to calculate the blood cells. It is widely used in…

Q: A total of 1,000 cells were counted from 5 squares of the hemocytometer. Of these, 20 are trypan…

A: Hemocytometer is a device which is made up of a glass which is thick and a microscopic slide that…

Q: A further sample of blood was taken from the above individual and prepared for haemoglobin analysis.…

A: The volume of a blood sample taken = 18 ml Volume of anticoagulant = 2 ml Out of this 20 ml, 5 ml…

Q: What is the meaning of the different values given engraved on each objective?

A: Microscopes are used in viewing microscopic organisms, including bacteria, parasites, etc., and also…

Q: After incubation of the standard plate count plates, you are instructed to count CFU’s of countable…

A: The Standard plate count technique is adopted by the microbiologists to count the population density…

Q: During a flame test, why is it necessary to use a new splint for each element?

A: A splint is a thin wood/stick used commonly in laboratory for performing a flame test. The splint is…

Q: Discuss the human life cycle and the purpose of meiosis. (please keep it short) 4 - 5 senttence

A: Life cycle of human: Human beings are made up of a billions of cells. Such large number of cells…

Q: What is a threshold setting (also called a trigger) used for when acquiring flow cytometric data?…

A: A threshold setting is used to determine when data acquisition should start and stop.

Q: If the test samples are positive for the antigen in the ELISA we are using, what color will you see?…

A: ELISA:                     ELISA ~ Enzyme-Linked Immunosorbent Assay          In analytical…

Q: If you performed the serial dilution using 0.9ml of tapwater originally and mixed and transferred…

A: A serial dilution is one of the techniques which is useful in microbiology, serology, and other…

Q: The figures below are the results of flow cytometer analysis of a drugs (camptotechin) effect on…

A: Flow cytometry is a technique that can be used to detect apoptotic activity in a cell.  Apoptosis is…

Q: Suppose you have a suspension of HeLa cells with a cell count of 85,000 cellsper ml, and you want to…

A: HeLa cells were discovered in 1950s and is named after the women from whom these cells are taken…

Q: Upon dislodging cells from the plate, you had the following cell counts using 4 different squares of…

A: Hemocytometer: It is a device first invented by " Louis charles malassesz". It is used to count the…

Q: ow do you figure out a number of live cells using flow cytometry results?

A: Flow Cytometry is a technique used to determine and analyze the characteristics of cells using…

Q: Why is UV light used in Column chromatography experiment ?

A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…

Q: when viewing an organism under the 40x objective lens, you estimate that 6 organims could fit across…

A: DFV stands for dimension of field of view. You'll need to determine the magnification and field…

Q: Describe disk diffusion test and how the data obtained from this test is interpreted (hint: think of…

A: Disk diffusion test - Disk diffusion is a microbiological technique used to determine the…

Q: The third micrograph is at the lowest magnification. Identify A (name of the cells; blank 1).…

A: These micrographs are of the organ :-  Spinal cord

Q: Draw cells in 4 large squares of the hemocytometer showing: 48 total viable cells (hollow dots) 9…

A: Hemocytometer is a counting- device used for counting blood cells. It is a glass slide with a cover…

Q: What is the principle of counting with a hemocytometer? What are the advantages and disadvantages?

A: Answer: HEMOCYTOMETER : It is the tool used in most of the biochemistry and biology labs in order to…

Q: Using the date in the photo of the two syringes below, answer these six questions: QUESTIONS 1.…

A: The given data and the graph represent the relationship with the photosynthesis process. The…

Q: syringes below, answer these six questions: QUESTIONS 1. Which syringe had the most floating disks?…

A: By the data given in the question we can see that this experiment is very much related to…

Q: There are five spicule specimens, identify the specific type of spicule

A: Spicules are the skeleton which can be calcareous or siliceous or fibers composed of spongin that…

Q: draw the results of a flow cytometry experiment in which one set of cells is treated with vehicle…

A: Taxol is a microtubule-stabilizing drug commonly utilized to treat cancer and can actuate cell cycle…

Hematology

Hematology is the branch of biology. Hematology is the medical field that deals with the treatment of blood disorders and cancers, such as hemophilia, leukemia, lymphoma, and sickle-cell anemia. Hematologists and hematopathologists are medical professionals who specialize in illnesses of the blood and its components.