If the DNA was replicated using the dispersive model, what would you have expected to observe in the generation 2 sample? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a b с d e Question 6 Homework Answered f g 3 distinct bands (one 15N, one 14/15N, and one 14N) 2 distinct bands (one 15N and one 14N) 2 distinct bands (one 15N and one 14/15 hybrid) Only one distinct band (15N) Only one distinct band (14/15 hybrid) Only one distinct band (14N) No bands

Give an estimate of the fragment sizes (basepairs) of the DNA bands from each sample lane of the gel electropherogram below. lane 1: lane 2: lane 3:

Please answer both parts, a. The enzyme that catalyzes the joining of fragments to form recombinant DNA is: Question 21 options: DNA polymerase DNA ligase helicase restriction endonuclease b. In the above diagram, ‘1’ represents the Question 3 options: sticky ends restriction sites primer restriction fragments

The following question is related to Restriction Enzymes and RFLP. Using EcoRl, the smallest fragments between DNA sequence A and B were how many base pairs?

This individual's x-ray diffraction data helped to confirm that DNA was orientied in a helical structure with uniform diameter. O a. Rosalind Franklin b. James Watson O c. Francis Crick O d. Gregor Mendel e. Edwin Chargaff

As the leading scientist in a biomedical science laboratory, it is a requirement to give advice to your lab assistants when they are having problems with their experiments. What advice would you give to your assistants that are having the following problems: After performing a polymerase chain reaction (PCR) and agarose gel electrophoresis to confirm the presence of the C01 gene of 750bp. 2.1. They observe no band appearing on an agarose gel. What would be your conclusion? 2.2. They observe three bands of different sizes that resemble a smear on the gel. Advice 2.3. They observe a single band on the gel and conclude that the PCR product is an exact copy of the original template DNA. Would you support their condusion? Explain. 2.4. Explain how PCR can be used to detect infectious agents in diagnoses of diseases.

A linear DNA fragment was produced by digestion with the restriction enzyme, Xba1. This fragment with XbaI(X) sites on both ends was then further digested with HindIII (H) and EcoRI (E).  Draw a restriction map of the linear fragment based on the gel electrophoresis results shown below.  X         H         Marker             E          H/E                           __2000bp __                    __1500b                         __1300bp        __             __        __1000bp                    __                         __700bp             ­­__        __500bp                         __400bp                         __300bp                      __                         __200bp          __        __                         __100bp

DNA samples were then run in agarose gel electrophoresis against a molecular standard of 100 bp and 1Kb Ladder. The figure is analyzed and labelled manually and recorded with the used product ladder information. Figure 2A is labeled with corresponding lane numbers and with M1 and M2 as markers used. Write an interpretation of the documented gelresult (Figure 2).  Note: Agarose gel of isolated DNA (A) from plant (lanes 1 & 2), chicken (lanes 3 & 4), and E. coli (lanes 5 & 6) with Vivantis* product information of 100bp (B) and 1kb ladders (C).

In E. coli, the DNA helicase is loaded onto the DNA by __________ and activated when __________ binds the helicase.   Question 24 options:   DnaC; DnaG   DnaA; DnaB   DnaG; DnaC   DnaB; DnaA   DnaC; DnaB

The results of gel electrophoresis of 4 different DNA samples given in the figure. 16 ul was loaded into each well (6 μL diluted DNA* + 8 μL water + 2 μL sample buffer). The ladder is in lane 5, with the size and amount (ng) in each band indicated. *1:5 dilutions of the original DNA sample were made, and the diluted samples were used for AGE. Q. These are the conclusions made about sizes and concentrations of the original (undiluted) DNA samples from lanes 1 and 2. Are these conclusions reasonable? Explain specifically, and if any of the conclusions are not reasonable, explain why not and what should we conclude instead. Sample in lane 1:size is 2.32 kb  concentration of original DNA sample is 9.2 ng/ul Sample in lane 2:size is 4.36 kb  concentration of original DNA samples is 100 ng/ul Q. The sample in lane 3 was expected to be about 2 kb in size. What is a possible explanation for the results observed?