Identify and mark each of the following on the portion of DNA undergoing replication: replication fork, DNA polymerase, RNA primer, parent strands, leading strand, lagging strand, the direction of replication on each strand, and the 5' end of each strand.

Does E. coli chromosomal replication always start at one particular site? What is called? If you were given the DNA sequence of E. coli chromosome, would you be able to identify where E. coli chromosomal replication starts? What is the end of E. coli chromosome replication?

Using the illustration of DNA replication given below label the following:

Spontaneous deamination of cytosine bases in DNA takes place at low but measurable frequency. Cytosine is converted into uracil by loss of its amino group. After this conversion, which base pair occupies this position in each of the daughter strands resulting from one round of replication? Two rounds of replication? (a) How many different 8-mer sequences of DNA are there? (Hint: There are 16 possible dinucleotides and 64 possible trinucleotides.) We can quantify the information- carrying capacity of nucleic acids in the following way. Each position can be one of four bases, corresponding to two bits of information (2² = 4). Thus, a chain of 5100 nucleotides corresponds to 2 × 5100 = 10,200 bits, or 1275 bytes (1 byte =8 bits). (b) How many bits of information are stored in an 8-mer DNA sequence? In the E. coli genome? In the human genome? (c) Compare each of these values with the amount of information that can be stored on a computer compact disc, or CD (about 700 megabytes).

As the strands are synthesized in replication, which of the following is true?   the leading strand is synthesized continuously from the 5' to 3' direction while the other strand is synthesized discontinuously in the 3' to 5' direction   both strands are synthesized  in the 5' to 3' direction   the strand that shows the Okazaki fragments is synthesized in the 3'-5' direction.   both strands are synthesized continuously

Semi-discontinuous replication means that:     DNA synthesis only occurs in the same direction as the replication fork.     the double helix unwinds and each strand serves as a template for a new DNA molecule.     there are Okazaki fragments associated with the leading strand.     DNA synthesis occurs in both a 5’ to 3’ and a 3’ to 5’ direction.     there is both a leading and a lagging strand associated with each replication fork.

Why does DNA have a leading strand and a lagging strand during replication?Select the correct row below that fills in the following blanks to answer the question above.___1st___ has to attach the new free-floating nucleotides to the ___2nd___ of the newly formed strand. The two strands of a DNA molecule are antiparallel, so in order for the lagging strand to be formed in the same direction, it has to be done in Okazaki fragments going in a ___3rd___ direction. Select one: a. 1st 2nd 3rd DNA polymerase 3' end 5' to 3' b. 1st 2nd 3rd DNA polymerase 5' end 3' to 5' c. 1st 2nd 3rd DNA ligase 3' end 5' to 3' d. 1st 2nd 3rd DNA helicase 5' end 3' to 5'

Define DNA replication/synthesis and semiconservative replication.  In addition, describe and/or define the role(s) of each of the following in the process of DNA replication/synthesis: DNA template strand, 5’ and 3’ ends, DNA helicase, DNA polymerase, single-strand binding proteins, topoisomerase, primase, Okazaki fragments, leading strand and lagging strand.

Which of the following statements is false about DNA replication? A) Each lagging strand of DNA is started by an RNA primer. B) DNA polymerase joins nucleotides in the 5' to 3' direction only. C) The leading strand of DNA is made continuously. D) More than one replication fork is present. E) A replication bubble opens in one direction only.

A region of DNA has six copies of a trinucleotide repeat. During one round of replication, the template strand slips as shown in the diagram. How many repeats will the DNA have if the newly synthesized strand is used as a template in the next round of replication? 1 5'-CAG 3'-GTC GTC 4 2 GTCH 3 2 CAG CAG GTC 3 GTC 5 4 CAG-3' GTC -5' 6 Next round of DNA replication