I. Unwinding of DNA and formation of replication fork II. Proofreading of strands and replacing any errors during the synthesis. III. Synthesizing a new strand that matches the template, by extending the primer via the addition of he nucleotides to the 3' end. IV. Formation of semiconservative strands Which stage use/s DNA polymerase? A. I and II B. II and III C. Il only D. Ill only Which of the following sequence shows the correct process of DNA replication? A. I, I, III, IV B. I, II, IV, III C. I, III, IV, II D. I, III. II, IV

I. Unwinding of DNA and formation of replication fork II. Proofreading of strands and replacing any errors during the synthesis. III. Synthesizing a new strand that matches the template, by extending the primer via the addition of he nucleotides to the 3' end. IV. Formation of semiconservative strands Which stage use/s DNA polymerase? A. I and II B. II and III C. Il only D. Ill only Which of the following sequence shows the correct process of DNA replication? A. I, I, III, IV B. I, II, IV, III C. I, III, IV, II D. I, III. II, IV

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter9: Molecular Biology

Section: Chapter Questions

Problem 5RQ: The initial mechanism for repairing nucleotide errors in DNA is _____. a. mismatch repair b. DNA...

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a. Pfu Polymerase b.dNTPs c.Buffer Match each component above to the correct function(s) listed below. Write your selection(s) for each component. You may have more than one answer for each. 1. unwinds DNA 2. synthesizes new DNA strands 3. enzymatically catalyzes Quikchange 4. nucleotide source for new DNA strands 5. Energy source for reaction(s) 6. Repairs errors in base pair matching 7. Maintains pH and salt levels 8. Creates polymer chains
Match these replication associated terms with the appropriate definition or function. L Separates DNA strands to expand the replication bubble. Uses template strand to synthesize complete RNA transcripts. II. DNA polymerase . Cleaves the bond between two adjacent nucleotides in a strand. III. Primase DNA consensus sequence at the 5' end of a gene where synthesis begins. IV. Ligase DNA sequence where replication will be initiated. V. V. Synthesizes leading and lagging strands. Helicase VIL Synthesizes RNA primers to initiate strand synthesis. origin VIL Covalently links the DNA backbones of Okazaki fragments.
III. DOUBLE MATCHING TYPE choose the enzyme/protein/requirement in Column A, and the processes/stages where it occurs in COLUMN B. Column A Column B Enzyme/Proteins/ Requirements Processes/Stages A. RNA polymerase B. DNA polymerase I C. DNA polymerase III D. Single strand binding protein E. Sigma factor F. Ligase G. Peptidyl transferase L Replication initiation i. Replication elongation iI. Transcription initiation iv. Transcription elongation v. Transcription termination vi. Activation of amino acids vii. Translation initiation viii. Translation elongation ix. Translation termination H. Primers I. Aminoacyl synthetase J. Gyrase K. Release factors L Rho factor M. AUG-fmet N. Initiation factors
Which of the following molecules has RNA-dependent DNA polymerase activity? A. DNA polymerase I B. DNApolymeraseIII C. RNApolymeraseD. LigaseE. Telomerase
DNA polymerase I functions in DNA replication to: O a. synthesize the RNA primer O b. O C. bind to single-stranded DNA and prevent it from reannealing O d. removes the RNA primers and fill in the gaps synthesize the leading and lagging strands synthesize the leading strand, but not the lagging strand e.
QUES Place the following enzymes in the correct order, in which they function in DNA replication: DNA polymerase Helicase Ligase Primase a. Helicase, Primase, DNA polymerase, Ligase b. Ligase, Helicase, Primase, DNA polymerase O c. Ligase, Primase, Helicase, DNA polymerase O d. Primase, Ligase, Helicase, DNA polymerase
Indicate the stage of DNA replication when each of thefollowing enzymes is active:a. helicaseb. primasec. DNA polymerasesd. ligasee. topoisomerasef. DNA gyrase
What does it mean to say thhat extension by DNA polymerase III proceed 5'---3'? A. The 5' end of a DNA polymerase molecule attaches to the 3' end of primase. B. DNA polymerase adds nucleotides to a growing strand, moving in the 5' to 3' direction. C. DNA polymerase seals nicks as it moves along a DNA strand toward the 3' end. D. DNA polymerase can only synthesize DNA at the 5' end of an existing strand of DNA.
The DNA polymerase I uses its to remove the RNA primers during DNA replication in E. coli cells. Select one: a. 3' to 5' exonuclease activity O b. 3' to 5' DNA helicase activity O c. 5' to 3' exonuclease activity O d. 5' to 3' DNA polymerase activity
catalyzes the cleavage of DNA at a specific ii fragments. The statement given above is completed nucleotide sequence. splices DNA into by the information in row Row i ii A. DNA polymerase DNA ligase B. restriction DNA ligase endonuclease DNA Ligase restriction endonuclease restriction endonuclease С. D. restriction endonuclease A OB C D
1. DNA polymerase A. Short DNA sequences synthesized on the lagging strand 2. replication fork B. Replicated discontinuously in the 5' to 3' direction 3. Chromosome C. The structure made of DNA that carries genes 4. free nucleotides D. The Cs, Gs, Ts, & As ready to be added to growing chain 5. Topoisomerase F. The Y-shaped point of origin for DNA replication 6. Okazaki Fragment G. Manages the overwinding of DNA ahead of the fork 7. Leading strand H. Lays down nucleotides in DNA replication 8. Lagging strand I. replicated continuously in the 3' - 5' direction
Some Steps Involved in Creating Recombinant DNA 1. Plasmid DNA is cut at the restriction site.2. Foreign DNA is cut at the restriction site.3. Plasmid DNA is opened and sticky ends are formed.4. Foreign DNA restriction fragments are isolated5. Target sequence in the plasmid is recognized by the restriction endonuclease.6. Target sequences in the foreign DNA are recognized by the restriction endonuclease.7. Foreign DNA restriction fragment is inserted into the plasmid at the restriction site.8. DNA ligase splices the foreign restriction fragment and the plasmid together. Identify the correct sequence of steps involved in cutting and reassembling DNA molecules to make recombinant DNA. Start with preparation of the plasmid DNA. Select one: a. 6, 2, 4, 7, 8, 5, 1, 3 b. 5, 1, 3, 6, 2, 4, 8, 7 c. 5, 1, 3, 6, 2, 4, 7, 8 d. 6, 2, 4, 5, 1, 3, 8, 7