How would you devise a screen to identify recessive mutations in Drosophila that result in embryo lethality? How would you propagate the recessive mutant alleles?

How does the presence of epigenetic modifications affect the accuracy and reliability of genetic analysis techniques such as DNA sequencing and PCR (Polymerase Chain Reaction)?

ow does DNA methylation occur? What enzyme is involved in DNA methylation? What are the consequences of DNA methylation? What is base flipping and why is it significant? How do bacteria use methylation to protect their own DNA? How is methylation is used to silence genes in plants and mammals? What is epigenetic silencing? How is DNA methylation involved in X-chromosome inactivation and genomic imprinting in mammals? What are boundary elements and what role do they play in separating chromatin domains? How do special DNA sites facilitate the transition between euchromatin and heterochromatin? How do boundary elements affect chromatin modification? How do boundary elements function by binding specific proteins? What are the elements required for chromosome function? List the specialized regions that direct essential chromosomal functions. How does the “origin” site initiate DNA replication? Why is a replication of linear DNA more complicated than the replication of circular DNA?

What would you predict to be the phenotype of a Drosophila larva whose mother was homozygous for a loss-of-function allele in the nanos gene?