Consider a bacterium that can synthesize an imaginary amino acid called fictamine. Initially, mutagenesis revealed three genes (X, Y, Z) that participate in the synthesis of fictamine. Two of these genes encode enzymes (called 1 and 2) that synthesize fictamine. The third is a regulatory gene. Mutations in the three genes gave the following phenotypes. Assume that all mutations are recessive. ++ = enzyme present; - - = enzyme absent enzyme activity genotype without fictamine with fictamine 1 2 strain X Y Z 1 2 wild-type + + + mutant a + + mutant b + + ++ mutant c + + ++ For each question below, include a one sentence explanation of your answer. i) Which gene encodes enzyme 1? ii) Which gene encodes enzyme 2? iii) Which gene encodes a regulator? iv) Is the regulator a positive or negative regulator of the genes encoding enzymes 1 and 2? v) Does the presence of fictamine activate or inactivate the regulatory molecule? ++ ++

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Consider a bacterium that can synthesize an imaginary amino acid called fictamine. Initially,
mutagenesis revealed three genes (X, Y, Z) that participate in the synthesis of fictamine.
Two of these genes encode enzymes (called 1 and 2) that synthesize fictamine. The third is a
regulatory gene. Mutations in the three genes gave the following phenotypes. Assume that
all mutations are recessive. ++ = enzyme present; - - = enzyme absent
enzyme activity
genotype
without fictamine
with fictamine
1
2
strain
X
Y
Z
1
2
wild-type +
+
+
++
++
mutant a
+
+
mutant b
+
+
++
mutant c
+
+
++
For each question below, include a one sentence explanation of your answer.
i) Which gene encodes enzyme 1?
ii) Which gene encodes enzyme 2?
iii) Which gene encodes a regulator?
iv) Is the regulator a positive or negative regulator of the genes encoding enzymes 1 and 2?
v) Does the presence of fictamine activate or inactivate the regulatory molecule?
1
1
Transcribed Image Text:Consider a bacterium that can synthesize an imaginary amino acid called fictamine. Initially, mutagenesis revealed three genes (X, Y, Z) that participate in the synthesis of fictamine. Two of these genes encode enzymes (called 1 and 2) that synthesize fictamine. The third is a regulatory gene. Mutations in the three genes gave the following phenotypes. Assume that all mutations are recessive. ++ = enzyme present; - - = enzyme absent enzyme activity genotype without fictamine with fictamine 1 2 strain X Y Z 1 2 wild-type + + + ++ ++ mutant a + + mutant b + + ++ mutant c + + ++ For each question below, include a one sentence explanation of your answer. i) Which gene encodes enzyme 1? ii) Which gene encodes enzyme 2? iii) Which gene encodes a regulator? iv) Is the regulator a positive or negative regulator of the genes encoding enzymes 1 and 2? v) Does the presence of fictamine activate or inactivate the regulatory molecule? 1 1
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