Calculate the pH of the solution that results following addition of 10 mL of 1 M NaOH to 40 mL of 1 M NH4Cl

You have been provided with stock solutions of: stock A:     0.06 M sodium pyrophosphate buffer pH 8.5 stock B:     3 M ethanol stock C:     0.015 M NAD+ stock D:     milli Q water Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e. volume of stock A = _________mL volume of stock B = _________mL volume of stock C = _________mL volume of stock D = _________mL Show your calculations to arrive at your answers.

Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.

Which of the following combinations would be the best choice to buffer the pH of a solution at approximately 7? Ionization Constants for Aqueous Weak Acids at 25 °C Acid K. (pK,) Conjugate Base Hydrogen phthalate ion, Cg H4(CO2H)(CO2) Acetic acid, CH3 CO,H Acetate ion, CH; CO2- 1.8 x 10-5 (4.74) Weak Acid Phthalic acid, C6 H4 (CO,H)2 1.3 x 10-3 (2.89) Dihydrogen phosphate Hydrogen phosphate ion, H2 PO4 Hydrogen phosphate ion, HPO42 6.2 x 10-8 (7.21) ion, HPO,2- Phosphate ion, PO,* 3.6 x 10-13 (12.44) O Na, HPO4 and NagPO4 O NaH2 PO4 and NazHPO4 O H;PO4 and NaH2 PO4