Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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You are studying a strain of bacteria that carries a temperature-sensitive mutation in one of the genes required for DNA replication. The bacteria grow normally at the lower temperature, but when the temperature is raised they die. When you analyze the remains of the bacterial cells grown at the higher temperature you find evidence of partly replicated DNA. When the strands of this DNA are separated by heating, numerous single-stranded DNA molecules around 1000 nucleotides long are found. Which of the proteins listed below are most likely to be impaired in these mutant bacteria and why?: Primase, Helicase, or DNA Polymerase?
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- An Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that the DNA remains double- stranded at the replication forks. What is the likely target affected by the temperature-sensitive mutation? DNA Ligase DNA polymerase lII primase DNA gyrase Single-stranded DNA-binding protein (SSB)arrow_forwardWhen you have prokaryotic replication, there is a short window (1-2 min.) where one strand of the DNA is not methylated (the DNA is hemi-methylated). This could help DNA repair mechanisms to know which strand are old or new. They could repair the new one with the old one as a guide. True Falsearrow_forwardA bacterium synthesizes DNA at each replication fork at a rate of 1500 nucleotides per second. If this bacterium completely replicates its circular chromosome by theta replication in 20 minutes, how many base pairs of DNA will its chromosome contain?arrow_forward
- Give typing answer with explanation and conclusionarrow_forwardDNA replication is vital for successful cell division. Explain the process of DNA replication. Make sure to use the following terms: helicase, S-phase, DNA polymerase and DNA ligase, template, free nucleotides. Use the diagram if it helps you illustrate your points.arrow_forwardA student was analyzing the base composition of a DNA sample, but has lost all the data except the content of Cytosine, which is 20%. Based on this information, reconstruct the missing data and list the base composition of the DNA sample.arrow_forward
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