What can you say based on a comparison of lanes 2 and 3? O The band labeled DNA is indeed DNA O The DNase does not digest tRNA The extract does not contain any endogenous nucleases O Incubation at 60°C was sufficient to denature the DNase

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1 2 3 4
Well
DNA
rRNA
tRNA
In the gel above, lanes 1, 2, and 3 contain the
bacterial cell extract and lane 4 contains tRNA.
DNase was added to the mixture in lanes 3 and
4: lane 1 was incubated at 0°C and lanes 2, 3,
and 4 were incubated at 37°C.
What can you say based on a comparison of
lanes 2 and 3?
The band labeled DNA is indeed DNA
The DNase does not digest tRNA
The extract does not contain any endogenous
nucleases
Incubation at 60°C was sufficient to denature the
DNase
Transcribed Image Text:1 2 3 4 Well DNA rRNA tRNA In the gel above, lanes 1, 2, and 3 contain the bacterial cell extract and lane 4 contains tRNA. DNase was added to the mixture in lanes 3 and 4: lane 1 was incubated at 0°C and lanes 2, 3, and 4 were incubated at 37°C. What can you say based on a comparison of lanes 2 and 3? The band labeled DNA is indeed DNA The DNase does not digest tRNA The extract does not contain any endogenous nucleases Incubation at 60°C was sufficient to denature the DNase
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