From the list given - choose all the regulatory sequences that you think would control the expression of this eukaryotic gene

Alternative splicing happens in both pro- and eukaryotes. True O False The yeast Gal80 protein inhibits transcription, but by binding to a transcriptional activator protein instead of to an operator. True O False

Which of the following parts of the eukaryotic promoter are bound by general transcription factors?   Group of answer choices A promoter proximal elements B enhancer elements C specific promoter elements D the core promoter E the distal promoter

Why must chromatin be remodeled at a eukaryotic promoter? Histone proteins must be added to the promoter so they can recruit RNA polymerase II Histone proteins are blocking promoter sequences recognized by general transcription factors (GTFs) Space needs to be made for the ribosome to bind to a nascent (i.e. newly transcribed) mRNA Because enhancer regions need to be exposed for general transcription factor binding To allow the spliceosome to remove exons from the mRNA during transcription

Which of the following general transcription factors aids polymerase binding in the right direction on promoters?  Group of answer choices TFIID TFIIB TFIIA TFIIF

Three overall methods of controlling EUkaryotic transcription are (check the correct 3 of these. Don't check more than 3.) using transcription factors denaturing proteins using operons DNA phosphorylation O DNA methylation chromatin remodeling controlling RNA stability O proofreading O performing ectopic recombination

Think of the eukaryotic gene expression process. Which column below accurately describes which components of the gene are translated by ribosomal complex? Component A В D Ribosomal binding site Yes No No Yes Promoter Yes Yes No No Exons Yes Yes Yes Yes Introns No Yes No No C O A

1. enzymes that catalyze histone acetlation are closesly associated with transription factors, which are proteins that promote transcription. why is this a good biochemical strategy? 2. sp1 is a sqeuence specific human DNA binding protein that binds to a region on the DNA called the GC box, a promoter element with the sequence GGGCGG. Binding of Sp1 to the GC box enhances RNA polymerase 2 activity 50- to a 100 fold. How would you use affinity chromatography to purify SP1?

1. What features of a protein-coding gene might be found in a non-coding gene? 2. What features are unnecessary in a non-coding gene?