From gene expression studies on development of the gut, you have decided to study a gene called Ulcer1. This gene is over expressed in people when they develop ulcers, but the cell types responsible for formation of ulcers are not known and if Ulcer1 is directly responsible for ulcer formation is not known. Results of a gene knock out of Ulcer1 in mice that was performed by another laboratory, revealed that conventional knock out of Ulcer1 is lethal shortly after birth. b. Describe an approach for determining which cell types express Ulcer1 using a transgenic approach with a reporter gene. Include a description of all elements of the vector that you will need

Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Promoter || Operator genC genB gen operon Regulator genA

The lactose operon in E. coli comprises two structural genes and three regulatory components.a.State the name of each structural gene and describe its function: b.) State the name of each regulatory component and describe its function

Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Question 1 Promoter Operator genC genB gen operon Regulator genA Transcription occurs when: A. The amino acid is not bound to the regulator, causing the regulator to bind to the operator. B. The amino acid is bound to the regulator, causing the regulator to bind to the operator. C. The amino acid is not bound to the regulator, causing the regulator to fail to bind to the operator. D. The amino acid is bound to the regulator, causing the regulator to fail to bind to the operator. E. The operon is…

Remember that hexose kinase is an enzyme required for hexose metabolism, a process that takes a 6-carbon sugar and breaks it down into 2 pyruvate molecules. You discover that rather than being hormonally regulated (as seen above in humans), hexose kinase is part of an operon in prokaryotic organisms. This operon is known as the "hexose operon" and it regulates expression of the complete set of enzymes needed to convert a hexose sugar into 2 pyruvate molecules. Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the control of its own promoter (see the schematic of the operon below). The hexose regulatory protein is sensitive to fatty acyl CoA levels. When all hexose fuel sources are depleted, the bacteria switch to lipid metabolism and fatty acyl CoA levels increase. This turns expression of the hexose operon off. +1 +1 Regulatory Gene Pregulator Poperon Hexose Operon Genes regulator promoter operon promoter Question 5 F) Complete the diagrams…

15c

Wnt4 is expressed in a variety of embryonic tissues. You decide to study this gene during mouse development Part 1) In the mouse embryo at gastrulations are Wnts concentrated at the anterior or posterior region? Part 2) You decide to conduct a Wnt4 immunoblot (Western blot) using tissues from mouse embryos. What key reagent do you need to carry out this method? Part 3) Your immunoblot shows that Wnt4 is expressed in the embryonic ovary. What also need to be expressed in the ovary for Wnt4 to have an effect there? Part 4) Outline in three dot points how Wnt4 can induce gene expression in neighbouring cells. Part 5) a. What method you would use to show that Wnt4 gene is required for ovary development in the mouse? b. In three dot point, outline how this method is carried out.

Explain please

Describe the regulation of expression of the lac Z, Y and A genes in E.coli when there is LOW glucose and HIGH lactose present in the cell.

The SIRT3 protein has been reported to increase the growth of certain types of cancer cells. Researchers studying the metabolism and development of stomach cancer cells investigated the potential role of the SIRT3 protein in these cells. In the first investigation, the researchers quantified the relative amount of SIRT3 in normal stomach cells and in each of four different lines of stomach cancer cells (Figure 1). Using data from a group of 100 patients with stomach cancer, the researchers also analyzed the relative amount of SIRT3 protein detected in samples of the cancer cells and in normal cells immediately adjacent to the cancer cells (Figure 2).     Figure 1. SIRT3 protein levels in normal stomach cells (NS) and stomach cancer cell lines from four different patients (SC-1, SC-2, SC-3, SC-4), shown relative to the level in normal stomach cells (NS). Error bars represent ±SEx¯.   Figure 2. The number of patients, from 100 tested, whose stomach cancer cells or normal stomach cells…