From gene expression studies on development of the gut, you have decided to study a gene called Ulcer1. This gene is over expressed in people when they develop ulcers, but the cell types responsible for formation of ulcers are not known and if Ulcer1 is directly responsible for ulcer formation is not known. Results of a gene knock out of Ulcer1 in mice that was performed by another laboratory, revealed that conventional knock out of Ulcer1 is lethal shortly after birth. b. Describe an approach for determining which cell types express Ulcer1 using a transgenic approach with a reporter gene. Include a description of all elements of the vector that you will need
From gene expression studies on development of the gut, you have decided to study a gene called Ulcer1. This gene is over expressed in people when they develop ulcers, but the cell types responsible for formation of ulcers are not known and if Ulcer1 is directly responsible for ulcer formation is not known. Results of a gene knock out of Ulcer1 in mice that was performed by another laboratory, revealed that conventional knock out of Ulcer1 is lethal shortly after birth.
b. Describe an approach for determining which cell types express Ulcer1 using a transgenic approach with a reporter gene. Include a description of all elements of the vector that you will need
Gene expression is the process where the information encoded in a gene is used to direct the assembly of a protein molecule. The cell reads the sequence of the gene in form of codons.
Gene expression starts when transcription begins, an RNA polymerase binds to the promoter sequence on the DNA molecule.
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