For what purposes is DNA extraction done? (give at least 3 purposes for which you may need to extract DNA)

What are the components require in a PCR reaction?  And justify them?  Please answer at your own words.

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What are some difficulties with low copy number DNA analysis? What kinds of checks and balances can a laboratory employ to ensure reliability of DNA profiles coming from low amounts of DNA template

Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing, with reference to a published protocol from a company that supplies regents and kits for DNA extraction (e.g Qiagen, ThermoFischer, Invitrogen, or similar). You should note the refinements compared to the protocol used in your practical session.

Please provide answers of both parts ..

Can you explain thie each of the statement given i dont really understand the dna recombinant is used as a molecular cloning and application for recombinant dna

Which of the following is not true about microarrays? Group of answer choices A microarray is a glass slide with single-stranded DNA attached to it. A microarray is covered in spots, where each spot represents a different sequence that will be assessed in the experiment. A microarray can assess hundreds of thousands of variants simultaneously. The same microarray slide can be used either for DNA microarrays or RNA microarrays

There are many PCR techniques available to suit the needs of all researchers in their laboratory task. (i) (ii) What is the major difference in the functions performed by the conventional PCR and real time PCR? Shania is planning to study the gene expression of Escherichia coli after a drug- treatment. She needs to decide between two types of chemistries to detect her PCR products (TaqMan Chemistry vs. SYBR Chemistry) using real-time PCR instruments. Compare and contrast between TaqMan Chemistry and SYBR Chemistry.

Microarray hybridization is used mostly in transcript profiling or assaying DNA variation.  Although the technology for establishing DNA microarrays was developed only recently, numerous applications have already been developed and their impact on future biomedical research and diagnostic approaches is expected to be profound.  Give some examples of the practical use of this technique.